Recombinant Anti-RASA1 antibody [EP536Y] - BSA and Azide free (ab247281)
![Western blot - Anti-RASA1 antibody [EP536Y] - BSA and Azide free (ab247281)](https://www.abcam.com/ps/products/247/ab247281/Images/ab247281-406320-anti-rasa1-antibody-ep536y-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-RASA1 antibody [EP536Y] - BSA and Azide free (ab247281)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP536Y] to RASA1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-RASA1 antibody [EP536Y] - BSA and Azide free
See all RASA1 primary antibodies -
Description
Rabbit monoclonal [EP536Y] to RASA1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human RASA1 aa 250-350 (internal sequence). The exact sequence is proprietary. From near the SH3 domain.
Database link: P20936 -
General notes
Ab247281 is the carrier-free version of ab40677. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247281 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP536Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab247281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 140 kDa (predicted molecular weight: 96 kDa). |
Target
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Function
Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21. -
Tissue specificity
In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level). -
Involvement in disease
Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.
Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.
Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 Ras-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5921 Human
- Entrez Gene: 218397 Mouse
- Entrez Gene: 25676 Rat
- Omim: 139150 Human
- SwissProt: P20936 Human
- SwissProt: P50904 Rat
- Unigene: 664080 Human
- Unigene: 12223 Rat
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Alternative names
- CM AVM antibody
- CMAVM antibody
- DKFZp434N071 antibody
see all
Images
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Western blot - Anti-RASA1 antibody [EP536Y] - BSA and Azide free (ab247281)Anti-RASA1 antibody [EP536Y] (ab40677) at 1/2000 dilution + Rat brain tissue lysate at 10 µg
Predicted band size: 96 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa (possible cleavage fragment)This data was developed using ab40677, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA1 antibody [EP536Y] - BSA and Azide free (ab247281)](https://www.abcam.com/ps/products/247/ab247281/Images/ab247281-1-anti-rasa1-antibody-ep536y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA1 antibody [EP536Y] - BSA and Azide free (ab247281)This data was developed using ab40677, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded human ovary carcinoma using ab40677 diluted 1:50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Anti-RASA1 antibody [EP536Y] - BSA and Azide free (ab247281)](https://www.abcam.com/ps/products/247/ab247281/Images/ab247281-3-benefits-of-recombinant-antibodies.png)
Anti-RASA1 antibody [EP536Y] - BSA and Azide free (ab247281)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab247281 has not yet been referenced specifically in any publications.
