Recombinant
RabMAb

Recombinant Anti-RASA1 antibody [EP536Y] - BSA and Azide free (ab247281)

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Overview

  • Product name

    Anti-RASA1 antibody [EP536Y] - BSA and Azide free
    See all RASA1 primary antibodies

  • Description

    Rabbit monoclonal [EP536Y] to RASA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human RASA1 aa 250-350 (internal sequence). The exact sequence is proprietary. From near the SH3 domain.
    Database link: P20936

  • General notes

    Ab247281 is the carrier-free version of ab40677. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247281 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EP536Y

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab247281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 140 kDa (predicted molecular weight: 96 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.

    • Tissue specificity

      In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level).

    • Involvement in disease

      Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.
      Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.
      Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb.

    • Sequence similarities

      Contains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 Ras-GAP domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.

    • Post-translational
      modifications

      The N-terminus is blocked.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • CM AVM antibody
        • CMAVM antibody
        • DKFZp434N071 antibody
        • GAP antibody
        • GTPase activating protein antibody
        • GTPase-activating protein antibody
        • OTTHUMP00000222390 antibody
        • OTTHUMP00000222391 antibody
        • OTTHUMP00000222392 antibody
        • OTTHUMP00000222393 antibody
        • p120GAP antibody
        • p120RASGAP antibody
        • PKWS antibody
        • Ras GTPase-activating protein 1 antibody
        • RAS p21 protein activator (GTPase activating protein) 1 antibody
        • Ras p21 protein activator antibody
        • RASA antibody
        • RASA1 antibody
        • RASA1_HUMAN antibody
        • RasGAP antibody
        • Triphosphatase activating protein antibody
        see all

      References

      ab247281 has not yet been referenced specifically in any publications.

      Publishing research using ab247281? Please let us know so that we can cite the reference in this datasheet.

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