Product nameRASA1 overexpression 293T lysate (whole cell)
ab94075 is a 293T cell transfected lysate in which Human GAP has been transiently over-expressed using a pCMV-GAP plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
Our Abpromise guarantee covers the use of ab94075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94075 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-RASA1 antibody (ab55663) at 1/500 dilution
Lane 1 :
RASA1 overexpression 293T lysate (whole cell) (ab94075)
Lane 2 : 293T non transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94075 has not yet been referenced specifically in any publications.