Key features and details
- Rabbit polyclonal to RASA2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RASA2 antibody
See all RASA2 primary antibodies
DescriptionRabbit polyclonal to RASA2
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- WB: A431 and PC3 cell lysates. IHC-P: Human small intestine, prostate and endimetrium tissue. ICC/IF: A431 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 97 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4).
Sequence similaritiesContains 1 Btk-type zinc finger.
Contains 2 C2 domains.
Contains 1 PH domain.
Contains 1 Ras-GAP domain.
Cellular localizationCytoplasm. Cytoplasm > perinuclear region.
- Information by UniProt
- GAP1m antibody
- GTPase activating protein of RAS antibody
- Ras GTPase activating protein 2 antibody
Paraffin-embedded human endometrium tissue stained for RASA2 using ab254590 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-RASA2 antibody (ab254590) at 0.4 µg/ml
Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : PC-3 cell lysate
Predicted band size: 97 kDa
PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling RASA2 using ab254590 at 4µg/ml (green) in ICC/IF.
Paraffin-embedded human prostate tissue stained for RASA2 using ab254590 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for RASA2 using ab254590 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for RASA2 using ab254590 at 1/50 dilution in immunohistochemical analysis. Low expression, as expected.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254590 has not yet been referenced specifically in any publications.