Product nameAnti-RASA3 antibody
See all RASA3 primary antibodies
DescriptionRabbit polyclonal to RASA3
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Xenopus laevis
Recombinant fragment corresponding to Human RASA3 aa 583-834 (C terminal).
Database link: Q14644
- WB: NT2D1, IMR32 and U-87 MG whole cell lysates. IHC-P: Human MCF7 xenograft tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab153846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Detects a band of approximately 96 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4) with high affinity. Might be a specific IP4 receptor.
Sequence similaritiesContains 1 Btk-type zinc finger.
Contains 2 C2 domains.
Contains 1 PH domain.
Contains 1 Ras-GAP domain.
Cellular localizationCell membrane.
- Information by UniProt
- GAP III antibody
- GAP1(IP4BP) antibody
- GAP1IP4BP antibody
All lanes : Anti-RASA3 antibody (ab153846) at 1/400 dilution
Lane 1 : Mouse platelet whole cell lysate
Lane 2 : Human platelet whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : IRDye 800CW - conjugated Oddyssey goat IgG at 1/15000 dilution
Performed under reducing conditions.
Additional bands at: 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 hour
Blocking agent: 3% BSA for 60 minutes at 20oC.
ab153846 staining RASA3 in a human megakaryocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 10% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/250) for 1 hour at 20°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/350) was used as the secondary antibody.
Anti-RASA3 antibody (ab153846) at 1/2000 dilution + IMR32 whole cell lysate at 30 µg
7.5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human MCF7 xenograft tissue labeling RASA3 with ab153846 at 1/100 dilution.
ab153846 has not yet been referenced specifically in any publications.