Key features and details
- Rabbit polyclonal to RASA4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RASA4 antibody
See all RASA4 primary antibodies
DescriptionRabbit polyclonal to RASA4
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 20-803 of Human RASA4.
- 293T, A431 and HeLa cell lysates; HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 90 kDa.|
|ICC/IF||1/100 - 1/1000.|
RelevanceRASA4 is a Ca(2+)-dependent Ras GTPase-activating protein, that switches off the Ras-MAPK pathway following a stimulus that elevates intracellular calcium.
Cellular localizationCytoplasm; cytosol. Cell membrane; peripheral membrane protein. Note=Localized to the cytosol as a result of its lack of phosphoinositide binding activity. Upon agonist-stimulated calcium mobilization, utilizes the C2A and C2B domains to associate with the plasma membrane.
- Ca2+ promoted Ras inactivator antibody
- Calcium promoted Ras inactivator antibody
- CAPRI antibody
All lanes : Anti-RASA4 antibody (ab151232) at 1/3000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 90 kDa
7.5% SDS PAGE
Immunofluorescent analysis of methanol-fixed HeLa cells labeling RASA4 with ab151232 at 1/500 dilution.
ab151232 has not yet been referenced specifically in any publications.