Key features and details
- Rabbit polyclonal to RASAL2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RASAL2 antibody
See all RASAL2 primary antibodies
DescriptionRabbit polyclonal to RASAL2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
antigen, corresponding to amino acids 860-969 of Human RASAL2
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 129 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
FunctionInhibitory regulator of the Ras-cyclic AMP pathway.
Sequence similaritiesContains 1 C2 domain.
Contains 1 PH domain.
Contains 1 Ras-GAP domain.
- Information by UniProt
- nGAP antibody
- NGAP_HUMAN antibody
- Ras GTPase-activating protein nGAP antibody
Immunocytochemistry/immunofluorescence analysis of U2OS cells labelling RASAL2 with ab121578 shows positive staining in the plasma membrane and focal adhesion sites.
All lanes : Anti-RASAL2 antibody (ab121578) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver
Lane 5 : Human tonsil
Predicted band size: 129 kDa
ab121578 has been referenced in 3 publications.
- Pan Y et al. RASAL2 promotes tumor progression through LATS2/YAP1 axis of hippo signaling pathway in colorectal cancer. Mol Cancer 17:102 (2018). PubMed: 30037330
- Fang JF et al. Upregulation of RASAL2 promotes proliferation and metastasis, and is targeted by miR-203 in hepatocellular carcinoma. Mol Med Rep 15:2720-2726 (2017). PubMed: 28447723
- Huang Y et al. RASAL2 down-regulation in ovarian cancer promotes epithelial-mesenchymal transition and metastasis. Oncotarget 5:6734-45 (2014). IHC ; Human . PubMed: 25216515