Recombinant Anti-CDC25 antibody [EPR1790(2)] (ab111830)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1790(2)] to CDC25
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CDC25 antibody [EPR1790(2)]
See all CDC25 primary antibodies -
Description
Rabbit monoclonal [EPR1790(2)] to CDC25 -
Host species
Rabbit -
Specificity
This antibody detects the band of interest in tissue lysates, but it did not detect the protein in cell lysate.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human hippocampus, hypothalamus, and midbrain lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1790(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 145 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 145 kDa. |
Target
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Function
Promotes the exchange of Ras-bound GDP by GTP. -
Sequence similarities
Contains 1 DH (DBL-homology) domain.
Contains 1 IQ domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 2 PH domains.
Contains 1 Ras-GEF domain. -
Domain
The DH (DBL-homology) domain mediates interaction with RASGRF2. -
Post-translational
modificationsUbiquitinated. - Information by UniProt
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Database links
- Entrez Gene: 5923 Human
- Entrez Gene: 192213 Rat
- Omim: 606600 Human
- SwissProt: Q13972 Human
- SwissProt: P28818 Rat
- Unigene: 459035 Human
- Unigene: 135776 Rat
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Alternative names
- CDC25 antibody
- CDC25L antibody
- GNRP antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab111830 has been referenced in 2 publications.
- Jiang T et al. ALOX12B promotes carcinogenesis in cervical cancer by regulating the PI3K/ERK1 signaling pathway. Oncol Lett 20:1360-1368 (2020). PubMed: 32724378
- Watts JM et al. A Case of AML Characterized by a Novel t(4;15)(q31;q22) Translocation That Confers a Growth-Stimulatory Response to Retinoid-Based Therapy. Int J Mol Sci 18:N/A (2017). WB ; Human . PubMed: 28696354