• Product name

  • Description

    Rabbit polyclonal to RASGRF2
  • Host species

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen, corresponding to internal sequence amino acids 702-807 of Human RASGRF2.

  • Positive control

    • Human colon tissue; Human cell line A-431; Human liver, Human tonsil, RT-4, and U-251 MG cell lysates.



Our Abpromise guarantee covers the use of ab121577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
WB 1/250 - 1/500.


  • Function

    Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation. Functions in synaptic plasticity by contributing to the induction of long term potentiation.
  • Tissue specificity

    Widely expressed with higher expression in brain, followed by heart, lung, pancreas and kidney. Detected in placenta. Expressed in brain and lung (at protein level).
  • Sequence similarities

    Contains 1 DH (DBL-homology) domain.
    Contains 1 IQ domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 2 PH domains.
    Contains 1 Ras-GEF domain.
  • Domain

    The Ras-GEF domain and the N-terminal Ras-GEF domain form a Ras-binding site and mediate Ras activation.
    The IQ domain mediates the calcium-dependent interaction with calmodulin but is dispensable for the Ras-GEF activity.
    The DH (DBL-homology) domain mediates interaction with RASGRF1 and EPB49 and is required for RAC1 activation.
  • Post-translational

    Phosphorylated by CDK5; down-regulates RASGRF2-mediated RAC1 activation.
    Ubiquitinated upon interaction with Ras. Ubiquitination leads to degradation through the 26S proteasome.
  • Cellular localization

    Cytoplasm. Cell membrane. Endoplasmic reticulum membrane. Translocates to membranes when activated. Found both at cell periphery and along the axon of neurons.
  • Information by UniProt
  • Database links

  • Alternative names

    • GRF2 antibody
    • Ras guanine nucleotide exchange factor 2 antibody
    • Ras protein specific guanine nucleotide releasing factor 2 antibody
    • Ras-GRF2 antibody
    • Ras-specific guanine nucleotide-releasing factor 2 antibody
    • RASGRF2 antibody
    • RGRF2_HUMAN antibody
    see all


  • ab121577, at a 1/150 dilution, staining RASGRF2 in paraffin-embedded Human colon tissue by immunohistochemistry.
  • All lanes : Anti-RASGRF2 antibody (ab121577) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique.
  • ab121577, at 4 µg/ml, staining RASGRF2 in PFA/Triton X-100 treated Human cell line A-431 by immunofluorescence. ab121577 staining is shown in green.


ab121577 has not yet been referenced specifically in any publications.

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