Key features and details
- Rabbit polyclonal to RASGRF2
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-RASGRF2 antibody
See all RASGRF2 primary antibodies
DescriptionRabbit polyclonal to RASGRF2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
Recombinant fragment within Human RASGRF2 (internal sequence). The exact sequence is proprietary.
Database link: O14827
- WB: Mouse brain lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 141 kDa.|
FunctionFunctions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation. Functions in synaptic plasticity by contributing to the induction of long term potentiation.
Tissue specificityWidely expressed with higher expression in brain, followed by heart, lung, pancreas and kidney. Detected in placenta. Expressed in brain and lung (at protein level).
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 IQ domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 2 PH domains.
Contains 1 Ras-GEF domain.
DomainThe Ras-GEF domain and the N-terminal Ras-GEF domain form a Ras-binding site and mediate Ras activation.
The IQ domain mediates the calcium-dependent interaction with calmodulin but is dispensable for the Ras-GEF activity.
The DH (DBL-homology) domain mediates interaction with RASGRF1 and EPB49 and is required for RAC1 activation.
modificationsPhosphorylated by CDK5; down-regulates RASGRF2-mediated RAC1 activation.
Ubiquitinated upon interaction with Ras. Ubiquitination leads to degradation through the 26S proteasome.
Cellular localizationCytoplasm. Cell membrane. Endoplasmic reticulum membrane. Translocates to membranes when activated. Found both at cell periphery and along the axon of neurons.
- Information by UniProt
- GRF2 antibody
- Ras guanine nucleotide exchange factor 2 antibody
- Ras protein specific guanine nucleotide releasing factor 2 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227009 has not yet been referenced specifically in any publications.