Overview

  • Product name

  • Description

    Rabbit polyclonal to RASGRF2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Recombinant fragment within Human RASGRF2 (internal sequence). The exact sequence is proprietary.
    Database link: O14827

  • Positive control

    • WB: Mouse brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab227009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 141 kDa.

Target

  • Function

    Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation. Functions in synaptic plasticity by contributing to the induction of long term potentiation.
  • Tissue specificity

    Widely expressed with higher expression in brain, followed by heart, lung, pancreas and kidney. Detected in placenta. Expressed in brain and lung (at protein level).
  • Sequence similarities

    Contains 1 DH (DBL-homology) domain.
    Contains 1 IQ domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 2 PH domains.
    Contains 1 Ras-GEF domain.
  • Domain

    The Ras-GEF domain and the N-terminal Ras-GEF domain form a Ras-binding site and mediate Ras activation.
    The IQ domain mediates the calcium-dependent interaction with calmodulin but is dispensable for the Ras-GEF activity.
    The DH (DBL-homology) domain mediates interaction with RASGRF1 and EPB49 and is required for RAC1 activation.
  • Post-translational
    modifications

    Phosphorylated by CDK5; down-regulates RASGRF2-mediated RAC1 activation.
    Ubiquitinated upon interaction with Ras. Ubiquitination leads to degradation through the 26S proteasome.
  • Cellular localization

    Cytoplasm. Cell membrane. Endoplasmic reticulum membrane. Translocates to membranes when activated. Found both at cell periphery and along the axon of neurons.
  • Information by UniProt
  • Database links

  • Alternative names

    • GRF2 antibody
    • Ras guanine nucleotide exchange factor 2 antibody
    • Ras protein specific guanine nucleotide releasing factor 2 antibody
    • Ras-GRF2 antibody
    • Ras-specific guanine nucleotide-releasing factor 2 antibody
    • RASGRF2 antibody
    • RGRF2_HUMAN antibody
    see all

Images

  • Anti-RASGRF2 antibody (ab227009) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 141 kDa



    5% SDS PAGE gel.

References

ab227009 has not yet been referenced specifically in any publications.

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