Product nameAnti-RASGRF2 antibody - C-terminal
See all RASGRF2 primary antibodies
DescriptionRabbit polyclonal to RASGRF2 - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
Recombinant fragment within Human RASGRF2 (C terminal). The exact sequence is proprietary.
Database link: O14827
- WB: Mouse and rat brain extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 141 kDa.|
FunctionFunctions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation. Functions in synaptic plasticity by contributing to the induction of long term potentiation.
Tissue specificityWidely expressed with higher expression in brain, followed by heart, lung, pancreas and kidney. Detected in placenta. Expressed in brain and lung (at protein level).
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 IQ domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 2 PH domains.
Contains 1 Ras-GEF domain.
DomainThe Ras-GEF domain and the N-terminal Ras-GEF domain form a Ras-binding site and mediate Ras activation.
The IQ domain mediates the calcium-dependent interaction with calmodulin but is dispensable for the Ras-GEF activity.
The DH (DBL-homology) domain mediates interaction with RASGRF1 and EPB49 and is required for RAC1 activation.
modificationsPhosphorylated by CDK5; down-regulates RASGRF2-mediated RAC1 activation.
Ubiquitinated upon interaction with Ras. Ubiquitination leads to degradation through the 26S proteasome.
Cellular localizationCytoplasm. Cell membrane. Endoplasmic reticulum membrane. Translocates to membranes when activated. Found both at cell periphery and along the axon of neurons.
- Information by UniProt
- GRF2 antibody
- Ras guanine nucleotide exchange factor 2 antibody
- Ras protein specific guanine nucleotide releasing factor 2 antibody
ab226973 has not yet been referenced specifically in any publications.