Overview

  • Product name
    Anti-RASGRP3 (phospho S172) antibody [EPNCIR149]
    See all RASGRP3 primary antibodies
  • Description
    Rabbit monoclonal [EPNCIR149] to RASGRP3 (phospho S172)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human RASGRP3.

  • Positive control
    • LNCap wild type cell lysates transfected with wild type RasGRP3, (C) wild type RASGRP3 + PMA (phorbol 12-myristate 13-acetate), wild type RASGRP3 + Go6983, wild type RASGRP3 + Go6983 + PMA
  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Peter Blumberg.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab166887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Guanine nucleotide exchange factor (GEF) for Ras and Rap1.
    • Sequence similarities
      Belongs to the RASGRP family.
      Contains 2 EF-hand domains.
      Contains 1 N-terminal Ras-GEF domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 Ras-GEF domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Calcium and DAG-regulated guanine nucleotide exchange factor III antibody
      • Grp3 antibody
      • GRP3_HUMAN antibody
      • Guanine nucleotide exchange factor for Rap1 antibody
      • Kiaa0846 antibody
      • RAS guanyl releasing protein 3 (calcium and DAG regulated) antibody
      • RAS guanyl releasing protein 3 calcium and DAG regualated guanine nucleotide exchange factor iii antibody
      • Ras guanyl-releasing protein 3 antibody
      • RASGRP3 antibody
      see all

    Images

    • All lanes : Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887) at 1/1000 dilution (purified)

      Lane 1 : LnCap wild type cell lysate transfected with RASGRP3 + Go6983
      Lane 2 : LnCap wild type cell lysate, untransfected

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 78 kDa
      Observed band size: 78 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887) at 1/1000 dilution

      Lane 1 : LNCap wild type
      cell lysate untransfected
      Lane 2 : LNCap wild type
      cell lysate transfected with RASGRP3
      Lane 3 : LNCap wild type
      cell lysate transfected with RASGRP3 + PMA
      Lane 4 : LNCap wild type
      cell lysate transfected with RASGRP3 + Go6983
      Lane 5 : LNCap wild type
      cell lysate transfected with RASGRP3 + Go6983 + PMA
      Lane 6 : LNCap wild type
      cell lysate transfected with s172A mutant RASGRP3
      Lane 7 : LNCap wild type
      cell lysate transfected with
      s172A mutant RASGRP3 + PMA
      Lane 8 : LNCap wild type
      cell lysate transfected with s172A mutant RASGRP3 + Go6983
      Lane 9 : LNCap wild type
      cell lysate transfected with s172A mutant RASGRP3 + Go6983 + PMA

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 78 kDa

    References

    ab166887 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab166887.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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