• Product name

    Anti-RASGRP3 (phospho T133) antibody [EPNCIR148]
    See all RASGRP3 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR148] to RASGRP3 (phospho T133)
  • Host species

  • Specificity

    ab124823 only detects RASGRP3 phosphorylated at Threonine 133.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Does not react with: Rat
  • Immunogen

    Phospho-specific peptide corresponding to residues surrounding Threonine 133 of Human RASGRP3.

  • Positive control

    • Ramos cell lysates
  • General notes

    This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Peter Blumberg. View antibodies from NCI Center for Cancer Research Collaboration.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab124823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Guanine nucleotide exchange factor (GEF) for Ras and Rap1.
    • Sequence similarities

      Belongs to the RASGRP family.
      Contains 2 EF-hand domains.
      Contains 1 N-terminal Ras-GEF domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 Ras-GEF domain.
    • Information by UniProt
    • Database links

    • Alternative names

      • Calcium and DAG-regulated guanine nucleotide exchange factor III antibody
      • Grp3 antibody
      • GRP3_HUMAN antibody
      • Guanine nucleotide exchange factor for Rap1 antibody
      • Kiaa0846 antibody
      • RAS guanyl releasing protein 3 (calcium and DAG regulated) antibody
      • RAS guanyl releasing protein 3 calcium and DAG regualated guanine nucleotide exchange factor iii antibody
      • Ras guanyl-releasing protein 3 antibody
      • RASGRP3 antibody
      see all


    • All lanes : Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823) at 1/1000 dilution

      Lane 1 : Ramos cell lysate, untreated

      Lane 2 : Ramos cell lysate treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Lane 1 : HRP labelled Goat anti-Rabbit at 1/2000 dilution
      Lane 2 : HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 78 kDa


    This product has been referenced in:

    • Tang S  et al. RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase. Nat Commun 5:4657 (2014). WB . Read more (PubMed: 25118589) »
    • Song X  et al. RasGRPs are targets of the anti-cancer agent ingenol-3-angelate. PLoS One 8:e72331 (2013). WB ; Rat . Read more (PubMed: 23991094) »
    See all 2 Publications for this product

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