Key features and details
- Rabbit polyclonal to RASL11A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RASL11A antibody
DescriptionRabbit polyclonal to RASL11A
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Human colon tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab180997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionRegulator of rDNA transcription. Acts in cooperation UBF/UBTF and positively regulates RNA polymerase I transcription.
Tissue specificityWidely expressed. Down-regulated in prostate tumors compared to normal prostate tissue. High levels found in colon tumor and normal colon tissue followed by small intestine, liver, jejunum, ileum, bladder and aorta. Lowest levels observed in endothelial cells.
Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
Developmental stageDown-regulated during development of THP-1 monocytes into macrophages.
Cellular localizationNucleus > nucleolus. Associates with rDNA transcription unit throughout the cell cycle.
- Information by UniProt
- Ras-like protein family member 11A antibody
- RAS-like, family 11, member A antibody
- Rasl11a antibody
- RSLBA_HUMAN antibody
Lanes 2-3 : Anti-RASL11A antibody (ab180997)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing RASL11A with a C-terminal myc-DDK tag (~3.1kDa)
Immunohistochemical analysis of paraffin-embedded Human colon tissue, labeling RASL11A using ab180997 at a 1/50 dilution.
ab180997 has not yet been referenced specifically in any publications.