Overview

  • Product name

  • Description

    Rabbit polyclonal to RASSF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RASSF1 aa 150-195 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    LFMSLNKDGSY TGFIKVQLKL VRPVSVPSSK KPPSLQDARR GPGRG


    Database link: Q9NS23-2

  • Positive control

    • Rat brain, human lung carcinoma and human oral squamous cell tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab203310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

WB 1/100 - 1/1000. Predicted molecular weight: 39 kDa.

Target

  • Function

    Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK4 during Fas-induced apoptosis. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.
  • Tissue specificity

    Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells.
  • Sequence similarities

    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-associating domain.
    Contains 1 SARAH domain.
  • Cellular localization

    Nucleus. Predominantly nuclear and Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Nucleus. Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 123F2 antibody
    • Cardiac specific ras association domain family 1 protein antibody
    • cardiac-specific ras association domain family 1 protein, 123F2 antibody
    • NORE2A antibody
    • OTTHUMP00000195503 antibody
    • OTTHUMP00000195504 antibody
    • OTTHUMP00000195505 antibody
    • OTTHUMP00000195507 antibody
    • Pancreas specific ras association domain family 1 protein antibody
    • Ras association (RalGDS/AF 6) domain family member 1 antibody
    • Ras association domain-containing protein 1 antibody
    • RASF1_HUMAN antibody
    • Rassf1 antibody
    • RASSF1A antibody
    • RDA32 antibody
    • REH3P21 antibody
    • Tumor suppressor protein RDA32 antibody
    • WUGSC:H_LUCA12.5 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human oral squamous cell tissue labeling RASSF1 with ab203310 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, FITC conjugated secondary antibody at 1/200 dilution for 40 minutes at 37°C.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human oral squamous cell tissue labeling RASSF1 with ab203310 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution for 40 minutes at 37°C.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling RASSF1 with ab203310 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling RASSF1 with ab203310 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

References

ab203310 has not yet been referenced specifically in any publications.

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