Overview

  • Product name

    Anti-RASSF1 antibody [EPR7127]
    See all RASSF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7127] to RASSF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human RASSF1 (Q9NS23).

  • Positive control

    • HeLa, Human fetal liver, HepG2, and 293T lysates; Human colon tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK4 during Fas-induced apoptosis. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.
    • Tissue specificity

      Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells.
    • Sequence similarities

      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 Ras-associating domain.
      Contains 1 SARAH domain.
    • Cellular localization

      Nucleus. Predominantly nuclear and Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Nucleus. Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 123F2 antibody
      • Cardiac specific ras association domain family 1 protein antibody
      • cardiac-specific ras association domain family 1 protein, 123F2 antibody
      • NORE2A antibody
      • OTTHUMP00000195503 antibody
      • OTTHUMP00000195504 antibody
      • OTTHUMP00000195505 antibody
      • OTTHUMP00000195507 antibody
      • Pancreas specific ras association domain family 1 protein antibody
      • Ras association (RalGDS/AF 6) domain family member 1 antibody
      • Ras association domain-containing protein 1 antibody
      • RASF1_HUMAN antibody
      • Rassf1 antibody
      • RASSF1A antibody
      • RDA32 antibody
      • REH3P21 antibody
      • Tumor suppressor protein RDA32 antibody
      • WUGSC:H_LUCA12.5 antibody
      see all

    Images

    • All lanes : Anti-RASSF1 antibody [EPR7127] (ab126764) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Human fetal liver cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 39 kDa

    • ab126764, at 1/50, staining RASSF1 in Paraffin-embedded Human colon tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Jia Y  et al. The different expression of tumor suppressors, RASSF1A, RUNX3, and GSTP1, in patients with alcoholic steatohepatitis (ASH) vs non-alcoholic steatohepatitis (NASH). Exp Mol Pathol 108:156-163 (2019). Read more (PubMed: 30951700) »
    • Zhang Y  et al. Prognostic and predictive role of COX-2, XRCC1 and RASSF1 expression in patients with esophageal squamous cell carcinoma receiving radiotherapy. Oncol Lett 13:2549-2556 (2017). IHC-P ; Human . Read more (PubMed: 28454432) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - other (293T)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-tris)
    Loading amount
    30 µg
    Specification
    293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Mar 12 2019

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