• Product name
    Anti-RASSF1a antibody [3F3]
    See all RASSF1a primary antibodies
  • Description
    Mouse monoclonal [3F3] to RASSF1a
  • Host species
  • Specificity
    In many cases, the results of staining showed speckled pattern or weak staining in cytoplasm but we couldn’t observe microtubule stained with this antibody. In mitotic cell, we could observe the mitotic spindles stained with this antibody and so thought it was kinetochore stained specifically.
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant human RASSF1A purified from E. coli.

  • Epitope
    ab23950 specifically recognises the C1 domain (52-101aa) of RASSF1a
  • Positive control
    • WB: HeLa lysate, 293cells transfected with HA-tagged RASSF 1A gene ICC: A431 cells
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.



Our Abpromise guarantee covers the use of ab23950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/500 - 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/200.
ICC/IF Use at an assay dependent concentration. Use at an assay dependent dilution. Use a biotinylated secondary antibody and Texas-red conjugated streptavidin. Use 4% paraformaldehyde as fixative for 20min and permeabilize in 0.4% Triton X-100.
Flow Cyt Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function
    Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK4 during Fas-induced apoptosis. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.
  • Tissue specificity
    Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells.
  • Sequence similarities
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-associating domain.
    Contains 1 SARAH domain.
  • Cellular localization
    Nucleus. Predominantly nuclear and Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Nucleus. Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ras association domain containing protein 1 antibody
    • Ras association domain family 1 antibody
    • Ras association domain family protein 1 antibody
    • Ras association domain-containing protein 1 antibody
    • RASF1_HUMAN antibody
    • RASSF 1a antibody
    • Rassf1 antibody
    • RASSF1A antibody
    • RASSF1A isoform antibody
    • Tumor suppressor protein RDA32 antibody
    see all


  • All lanes : Anti-RASSF1a antibody [3F3] (ab23950) at 1/1000 dilution

    Lane 1 : Hela (H) cell extract
    Lane 2 : Mouse brain (MB) extract
    Lane 3 : Mouse liver (ML) extract
    Lane 4 : Mouse kidney (MK) extract

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat anti-mouse HRP conjugate

    Developed using the ECL technique.

    Predicted band size: 39 kDa
    Observed band size: 40,43 kDa (why is the actual band size different from the predicted?)

    Arrows indicate RASSF1A protein (about 40kDa in Hela cells, and 43kDa in other tissues from mice).
  • A431 cells were labeled with anti-RASSF1a(clone 3F3) ab23950. Detection was done using a biotinylated secondary antibody and Texas-red conjugated streptavidin.
  • All lanes : Anti-RASSF1a antibody [3F3] (ab23950) at 1/1000 dilution

    Lane 1 : HEK293 cells
    Lane 2 : A549 cells
    Lane 3 : HeLa cells

    Predicted band size: 39 kDa

    Proteins were visualised using a goat anti-mouse secondary antibody conjugation to HRP and a ECL detection system.
  • Overlay histogram showing HeLA cells stained with ab23950 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab23950, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:
  • Ram RR  et al. RASSF1A inactivation unleashes a tumor suppressor/oncogene cascade with context-dependent consequences on cell cycle progression. Mol Cell Biol 34:2350-8 (2014). Read more (PubMed: 24732797) »
  • Gou HF  et al. Epidermal growth factor receptor (EGFR)-RAS signaling pathway in penile squamous cell carcinoma. PLoS One 8:e62175 (2013). IHC-P ; Human . Read more (PubMed: 23637996) »

See all 12 Publications for this product

Customer reviews and Q&As

The anti-RASSF1a ab23950 recognises the first exon and should therefore discriminate the exon1 deleted mutant to the wild type protein.

Thanks for your enquiry.
We do not have a specific protocol for cell extraction when preparing samples to western blot RASSF1a. We do have a good general WB protocol which you might find useful at https://www.abcam.com/index.html?pageconfig=resour...

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Thank you for sending this paper. I have passed this information on to the lab and my colleague provided the following clarification of our testing:

"During the development of this antibody, our western blot result wasn't good in A431 cells, ...

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Thank you for your enquiry. This antibody was raised to recombinant full-length hRASSF1a, and not a peptide sequence. The most detailed information we have regarding the epitope of ab23950 is that it specifically recognises the C1 domain (1-340aa) ...

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Thank you for providing the customer's detailed protocol, it enabled me to investigate the problem rapidly and thoroughly. There are two main points I would like to concentrate on: 1) levels of the protein may be low in the customer's samples. ...

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I have received feedback today from the laboratory that tested ab23950 and was informed that they have used A431 cells fixed with 4% PFA, methanol has not been tested. Cells were labeled with ab23950 and detection was achieved using a biotinylated ...

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Thank you for your enquiry. Following some epitope mapping by the lab I can confirm that Mouse monoclonal [3F3] to RASSF1a (ab23950) recognizes specifically the C1 domain(52-101aa) of RASSF1a. I hope this information helps, please do not hesitat...

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I have just received feedback from the source of ab23950 and have been informed that we are studying ab23950 with two university groups. Those groups used cell lysate of HeLa as positive control and 293cells transfected with HA-tagged RASSF 1A and ...

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Thank you for your patience and I'm sorry to hear that your customer is experiencing difficulty with this antibody. Our source for ab23950 was able to provide the following details regarding its characterization in Western blotting. The extracts of HeL...

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