Overview

  • Product name
  • Description
    Rabbit polyclonal to RASSF1a
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 50 and 334 of Human RASSF1a (NP_733832).

  • Positive control
    • A431 and HeLaS3 cell lines. 293T whole cell lysate. HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK4 during Fas-induced apoptosis. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.
  • Tissue specificity
    Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells.
  • Sequence similarities
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-associating domain.
    Contains 1 SARAH domain.
  • Cellular localization
    Nucleus. Predominantly nuclear and Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Nucleus. Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ras association domain containing protein 1 antibody
    • Ras association domain family 1 antibody
    • Ras association domain family protein 1 antibody
    • Ras association domain-containing protein 1 antibody
    • RASF1_HUMAN antibody
    • RASSF 1a antibody
    • Rassf1 antibody
    • RASSF1A antibody
    • RASSF1A isoform antibody
    • Tumor suppressor protein RDA32 antibody
    see all

Images

  • Anti-RASSF1a antibody (ab97749) at 1/500 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 39 kDa



    12% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97749 at 1/100 dilution. Lower image is merged with DNA probe.

References

This product has been referenced in:
  • Chen T  et al. Circ_0078767 suppresses non-small-cell lung cancer by protecting RASSF1A expression via sponging miR-330-3p. Cell Prolif N/A:e12548 (2018). Read more (PubMed: 30507050) »
  • Li W  et al. Suppressor of hepatocellular carcinoma RASSF1A activates autophagy initiation and maturation. Cell Death Differ N/A:N/A (2018). Read more (PubMed: 30315205) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

Thanks for your enquiry.
We do not have a specific protocol for cell extraction when preparing samples to western blot RASSF1a. We do have a good general WB protocol which you might find useful at https://www.abcam.com/index.html?pageconfig=resource&rid=11375.
Based on information on the datasheet for ab23950, there is western blot data showing detection of RASSF1a in Hela cells, mouse brain, liver and kidney extract.
Based on information provided in the SwissProt database, this protein has multiple forms but is expressed in all tissues, but often not in the corresponding tumor cell lines. It may be useful to check the literature on RASSF1a for cell lines that do contain detectable levels.
Since normal tissues commonly contain detectable levels of RASSF1a, you may be interested in our range of tissue extracts. Simply type in the tissue type into the product search bar at the top of our website, and select 'Lysates' as the product type.
Also, the SwissProt protein entry for RASSF1a notes that this protein can change cocalization, from cytoplsam to nuclear, depending on the cell cycle. Again, it may be useful to consult the literature for further details.
I hope this is helpful. Please contact us again if you have any further questions.

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