Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200
|WB||1/100 - 1/1000. Predicted molecular weight: 38 kDa.|
FunctionPotential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest.
Tissue specificityWidely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines.
Sequence similaritiesContains 1 Ras-associating domain.
Contains 1 SARAH domain.
- Information by UniProt
- CENP 34 antibody
- Centromere protein 34 antibody
- Ras association (RalGDS/AF 6) domain family 2 antibody
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat brain tissue sections labeling RASSF2 with ab203580 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
All lanes : Anti-RASSF2 antibody (ab203580) at 1/200 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
All lanes : Goat anti-rabbit IgG H+L HRP conjugated at 1/3000 dilution
Predicted band size: 38 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
ab203580 has not yet been referenced specifically in any publications.