Overview

  • Product name

  • Description

    Rabbit polyclonal to RASSF5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human RASSF5 aa 374-418 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    IPELQNF LTILEKEEQD KIQQVQKKYD KFRQKLEEAL RESQGKPG


    Database link: Q8WWW0

  • Positive control

    • IHC-P: Rat spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab218191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing. May be involved in regulation of Ras apoptotic function. The RASSF5-STK4 complex may mediate HRAS1 and KRAS induced apoptosis.
  • Tissue specificity

    Widely expressed. Frequently down-regulated in lung tumor cell lines and primary lung tumors.
  • Sequence similarities

    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-associating domain.
    Contains 1 SARAH domain.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Isoform 2 is mainly located in the perinuclear region of unstimulated primary T cells. Upon stimulation translocates to the leading edge and colocalizes with ITGAL/LFA-1 in the peripheral zone of the immunological synapse. Isoform 2 is localized to growing microtubules in vascular endothelial cells and is dissociated from microtubules by activated RAP1A.
  • Information by UniProt
  • Database links

  • Alternative names

    • Maxp 1 antibody
    • MAXP1 antibody
    • MGC10823 antibody
    • MGC17344 antibody
    • New ras effector 1 antibody
    • NORE 1 antibody
    • NORE1 antibody
    • NORE1A antibody
    • NORE1B antibody
    • Novel Ras effector 1 antibody
    • RAP1 Binding Protein antibody
    • RAPL antibody
    • Ras association (RalGDS/AF 6) domain family 5 antibody
    • Ras association (RalGDS/AF 6) domain family member 5 antibody
    • Ras association domain containing family protein 5 antibody
    • Ras association domain-containing protein 5 antibody
    • Ras effector like protein antibody
    • RASF5_HUMAN antibody
    • RASSF 3 antibody
    • RASSF 5 antibody
    • RASSF3 antibody
    • RASSF5 antibody
    • Regulator for cell adhesion and polarization enriched in lymphoid tissue antibody
    • Regulator for cell adhesion and polarization enriched in lymphoid tissues antibody
    • Tumor suppressor RASSF3 antibody
    see all

Images

  • Immunohistochemical analysis of paraformaldehyde fixed, paraffin embedded Rat spleen tissue labeling RASSF5 with ab218191 at 1/400 followed by a conjugated secondary for 20 minutes and DAB staining.

References

ab218191 has not yet been referenced specifically in any publications.

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