Recombinant Anti-RASSF8 antibody [EPR10239(2)] (ab175923)
![Western blot - Anti-RASSF8 antibody [EPR10239(2)] (ab175923)](https://www.abcam.com/ps/products/175/ab175923/Images/ab175923-202839-anti-rassf8-antibody-epr102392-western-blot.jpg "Western blot - Anti-RASSF8 antibody [EPR10239(2)] (ab175923)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10239(2)] to RASSF8
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-RASSF8 antibody [EPR10239(2)]
See all RASSF8 primary antibodies -
Description
Rabbit monoclonal [EPR10239(2)] to RASSF8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RASSF8 aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q8NHQ8-1 -
Positive control
- Hela and HT-1080 cell lysates, MCF-7 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10239(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab175923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 48 kDa. | |
| ICC/IF | 1/100 - 1/250. |
Target
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Tissue specificity
Widely expressed as a 6.2 kb transcript. A 2.2 kb alternatively spliced transcript is expressed exclusively in testis. -
Involvement in disease
Note=A chromosomal aberration involving RASSF8 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. -
Sequence similarities
Contains 1 Ras-associating domain. - Information by UniProt
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Database links
- Entrez Gene: 11228 Human
- Entrez Gene: 71323 Mouse
- Entrez Gene: 312846 Rat
- Omim: 608231 Human
- SwissProt: Q8NHQ8 Human
- SwissProt: Q8CJ96 Mouse
- Unigene: 696433 Human
- Unigene: 158669 Mouse
see all -
Alternative names
- C12orf2 antibody
- Carcinoma associated HOJ 1 antibody
- Carcinoma associated protein HOJ 1 antibody
see all
Images
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Western blot - Anti-RASSF8 antibody [EPR10239(2)] (ab175923)All lanes : Anti-RASSF8 antibody [EPR10239(2)] (ab175923) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HT-1080 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 48 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-RASSF8 antibody [EPR10239(2)] (ab175923)](https://www.abcam.com/ps/products/175/ab175923/Images/ab175923-202844-anti-rassf8-antibody-epr102392-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RASSF8 antibody [EPR10239(2)] (ab175923)Immunofluorescent staining of MCF-7 cells labeling RASSF8 with ab175923 at 1/100 dilution
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![Anti-RASSF8 antibody [EPR10239(2)] (ab175923)](https://www.abcam.com/ps/products/175/ab175923/Images/ab175923-3-benefits-of-recombinant-antibodies.png)
Anti-RASSF8 antibody [EPR10239(2)] (ab175923)
Protocols
Datasheets and documents
References (0)
ab175923 has not yet been referenced specifically in any publications.
