Anti-RASSF8 antibody [EPR10239(2)] (ab175923)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-RASSF8 antibody [EPR10239(2)]
See all RASSF8 primary antibodies -
DescriptionRabbit monoclonal [EPR10239(2)] to RASSF8
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RASSF8 aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q8NHQ8-1 -
Positive control
- Hela and HT-1080 cell lysates, MCF-7 cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR10239(2)
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab175923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 48 kDa. | |
| ICC/IF | 1/100 - 1/250. |
Target
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Tissue specificityWidely expressed as a 6.2 kb transcript. A 2.2 kb alternatively spliced transcript is expressed exclusively in testis.
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Involvement in diseaseNote=A chromosomal aberration involving RASSF8 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2.
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Sequence similaritiesContains 1 Ras-associating domain.
- Information by UniProt
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Database links
- Entrez Gene: 11228 Human
- Entrez Gene: 71323 Mouse
- Entrez Gene: 312846 Rat
- Omim: 608231 Human
- SwissProt: Q8NHQ8 Human
- SwissProt: Q8CJ96 Mouse
- Unigene: 696433 Human
- Unigene: 158669 Mouse
see all -
Alternative names
- C12orf2 antibody
- Carcinoma associated HOJ 1 antibody
- Carcinoma associated protein HOJ 1 antibody
see all
Images
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![Western blot - Anti-RASSF8 antibody [EPR10239(2)] (ab175923)](https://www.abcam.com/ps/products/175/ab175923/Images/ab175923-202839-wb1.jpg)
Western blot - Anti-RASSF8 antibody [EPR10239(2)] (ab175923)All lanes : Anti-RASSF8 antibody [EPR10239(2)] (ab175923) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HT-1080 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 48 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-RASSF8 antibody [EPR10239(2)] (ab175923)](https://www.abcam.com/ps/products/175/ab175923/Images/ab175923-202844-icc1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RASSF8 antibody [EPR10239(2)] (ab175923)Immunofluorescent staining of MCF-7 cells labeling RASSF8 with ab175923 at 1/100 dilution
Datasheets and documents
References
ab175923 has not yet been referenced specifically in any publications.
