Overview

  • Product name
    Rat Activin A ELISA Kit
    See all Activin A kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    2 pg/ml - 400 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum = 143.3 129% - 150%
    Plasma = 138.7 127% - 150%
    Cell culture media = 121.7 105% - 149%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Abcam’s Rat Activin A ELISA Kit (ab193733) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Activin A in sera, plasma and cell culture supernatants.

    This assay employs an antibody specific for rat Activin A coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Activin A present in the samples. The wells are washed and biotinylated anti-rat Activin A antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Activin A bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated Rat Activin A Detection Antibody (lyophilized) 2 vials
    Pre-coated rat Activin A Microplate (12 strips x 8 wells) 1 unit
    Rat Activin A Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules.1 Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types.2 Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
  • Cellular localization
    Secreted
  • Alternative names
    • Activin beta A chain
    • Beta 1
    • erythroid differentiation factor
    • erythroid differentiation protein
    • follicle stimulating hormone releasing protein
    • FSH releasing protein
    • Inhibin
    • inhibin beta A chain
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • A typical standard curve using ab193733 with Assay Diluent A

  • A typical standard curve using ab193733 with Assay Diluent B

Protocols

References

ab193733 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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