Anti-Rat Albumin antibody (FITC) (ab53435)

Reviews (1)Q&A (4)References (6)

Overview

  • Product name

    Anti-Rat Albumin antibody (FITC)

  • Description

    Sheep polyclonal to Rat Albumin (FITC)

  • Host species

    Sheep

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: IHC-Fr, ELISA, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Cow

  • Immunogen

    Full length native protein (purified) (Rat)

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...

  • Purification notes

    Antisera to rat albumin were raised by repeated immunisations of sheep with highly purified antigen. The purified IgG was prepared by affinity chromatography.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 19023867
ELISA 1/1000 - 1/25000.
ICC/IF 1/10 - 1/50.
IHC-P Use at an assay dependent concentration. PubMed: 24303036

Target

  • Function

    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.

  • Tissue specificity

    Plasma.

  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.

  • Post-translational
    modifications

    Phosphorylation sites are present in the extracelllular medium.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Alb 1 antibody
      • alb antibody
      • ALBU_RAT antibody
      • Albumin antibody
      • Serum albumin antibody
      see all

    References

    This product has been referenced in:

    • Kovacs ZI  et al. Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammation. Proc Natl Acad Sci U S A 114:E75-E84 (2017). Read more (PubMed: 27994152) »
    • Yan T  et al. Niaspan attenuates the adverse effects of bone marrow stromal cell treatment of stroke in type one diabetic rats. PLoS One 8:e81199 (2013). IHC-P ; Rat . Read more (PubMed: 24303036) »
    See all 6 Publications for this product

    Publishing research using ab53435? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Rat Albumin antibody (FITC)

     Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Frozen Liver Tissue Sections)
    Specification
    Frozen Liver Tissue Sections
    Fixative
    Methanol
    Permeabilization
    Yes - 0.3% Triton X-100
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 18 2010

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