|Sample type||Average %||Range|
|Serum||> 85||% - %|
Abcam’s Rat Alpha 1 Acid Glycoprotein (AGP) ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Alpha 1 Acid Glycoprotein levels in rat serum and plasma samples.
In this assay the Alpha 1 Acid Glycoprotein present in samples reacts with the anti-Alpha 1 Acid Glycoprotein antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Alpha 1 Acid Glycoprotein antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Alpha 1 Acid Glycoprotein. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 1 Acid Glycoprotein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 1 Acid Glycoprotein in the test sample. The quantity of Alpha 1 Acid Glycoprotein in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
|Components||1 x 96 tests|
|100X HRP-conjugated anti-rat Alpha 1 Acid Glycoprotein antibody||1 x 150µl|
|20X Wash Buffer Concentrate||1 x 50ml|
|5X Diluent Concentrate||1 x 50ml|
|Chromogen Substrate Solution||1 x 12ml|
|Rat Alpha 1 Acid Glycoprotein Calibrator (lyophilized)||1 unit|
|Rat Alpha 1 Acid Glycoprotein ELISA Microplate||1 unit|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab157729 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab157729 has not yet been referenced specifically in any publications.