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Rat alpha 2 Macroglobulin ELISA Kit (ab157730)

  • Datasheet
  • SDS
  • Protocol Booklet
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Sandwich ELISA - ab157730 alpha 2 Macroglobulin Rat Elisa kit
  • Standard Curve

Key features and details

  • Sensitivity: 2.23 ng/ml
  • Range: 6.25 ng/ml - 400 ng/ml
  • Sample type: Other biological fluids, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

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Overview

  • Product name

    Rat alpha 2 Macroglobulin ELISA Kit
    See all alpha 2 Macroglobulin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type

    Serum, Plasma, Other biological fluids
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2.23 ng/ml
  • Range

    6.25 ng/ml - 400 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s alpha 2 Macroglobulin Rat ELISA Kit is highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of alpha 2 Macroglobulin in rat biological samples. This kit is specific to the alpha 2 Macroglobulin and will not cross react with the closely related alpha 1 Macroglobulin. 

    In this assay the alpha 2 Macroglobulin present in samples reacts with the anti-alpha 2 Macroglobulin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-alpha 2 Macroglobulin antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound alpha 2 Macroglobulin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of alpha 2 Macroglobulin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of alpha 2 Macroglobulin in the test sample. The quantity of alpha 2 Macroglobulin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-conjugated anti-rat alpha 2 Macroglobulin antibody 1 x 150µl
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    Chromogen Substrate Solution 1 x 12ml
    Rat alpha 2 Macroglobulin Calibrator (lyophilized) 1 vial
    Rat alpha 2 Macroglobulin ELISA Microplate 1 unit
    Stop Solution 1 x 12ml
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Blood
    • Coagulation
    • Intrinsic
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Other protease inhibitors
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Complement ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Protease inhibitors ELISA kits
  • Function

    Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • Tissue specificity

    Secreted in plasma.
  • Sequence similarities

    Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • Developmental stage

    Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01023 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • A2m
    • A2MG_HUMAN
    • Alpha 2 M
    • Alpha 2M
    • Alpha-2-M
    • Alpha-2-macroglobulin
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
    • CPAMD5
    • DKFZp779B086
    • FWP007
    • S863 7
    see all
  • Database links

    • Entrez Gene: 24153 Rat
    • SwissProt: P06238 Rat
    • Unigene: 225884 Rat

    Associated products

      Images

      • Sandwich ELISA - ab157730 alpha 2 Macroglobulin Rat Elisa kit
        Sandwich ELISA - ab157730 alpha 2 Macroglobulin Rat Elisa kit

        alpha-2-macroglobulin measured in various samples showing quantitie (ng) per mL of tested sample

      • Standard Curve
        Standard Curve
        Representative standard curve using ab157730 alpha 2 Macroglobulin Rat ELISA Kit.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab157730? Please let us know so that we can cite the reference in this datasheet.

      ab157730 has been referenced in 1 publication.

      • Maldonado-Bouchard S  et al. Inflammation is increased with anxiety- and depression-like signs in a rat model of spinal cord injury. Brain Behav Immun 51:176-95 (2016). PubMed: 26296565

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