Rat alpha 2 Macroglobulin ELISA Kit (ab157730)
Overview
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Product name
Rat alpha 2 Macroglobulin ELISA Kit
See all alpha 2 Macroglobulin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10% -
Sample type
Serum, Plasma, Other biological fluids -
Assay type
Sandwich (quantitative) -
Sensitivity
2.23 ng/ml -
Range
6.25 ng/ml - 400 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s alpha 2 Macroglobulin Rat ELISA Kit is highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of alpha 2 Macroglobulin in rat biological samples. This kit is specific to the alpha 2 Macroglobulin and will not cross react with the closely related alpha 1 Macroglobulin.
In this assay the alpha 2 Macroglobulin present in samples reacts with the anti-alpha 2 Macroglobulin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-alpha 2 Macroglobulin antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound alpha 2 Macroglobulin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of alpha 2 Macroglobulin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of alpha 2 Macroglobulin in the test sample. The quantity of alpha 2 Macroglobulin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-conjugated anti-rat alpha 2 Macroglobulin antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Rat alpha 2 Macroglobulin Calibrator (lyophilized) 1 vial Rat alpha 2 Macroglobulin ELISA Microplate 1 unit Stop Solution 1 x 12ml -
Research areas
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Function
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. -
Tissue specificity
Secreted in plasma. -
Sequence similarities
Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. -
Developmental stage
Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- A2m
- A2MG_HUMAN
- Alpha 2 M
see all -
Database links
- Entrez Gene: 24153 Rat
- SwissProt: P06238 Rat
- Unigene: 225884 Rat
Images
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Sandwich ELISA - ab157730 alpha 2 Macroglobulin Rat Elisa kitalpha-2-macroglobulin measured in various samples showing quantitie (ng) per mL of tested sample
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Standard CurveRepresentative standard curve using ab157730 alpha 2 Macroglobulin Rat ELISA Kit.
References
ab157730 has been referenced in 1 publication.
- Maldonado-Bouchard S et al. Inflammation is increased with anxiety- and depression-like signs in a rat model of spinal cord injury. Brain Behav Immun 51:176-95 (2016). PubMed: 26296565
