Overview

  • Product name

    Rat Angiopoietin-1 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1.31ng/ml 0.046 3.5%
    2 16 3.64ng/ml 0.138 3.8%
    3 16 6.95ng/ml 0.292 4.2%
    Inter-assay
    Sample n Mean SD CV%
    1 24 1.42ng/ml 0.058 4.1%
    2 24 3.75ng/ml 0.173 4.6%
    3 24 7.03ng/ml 0.401 5.7%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    The Rat Angiopoietin-1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213920) is designed for the quantitative measurement of Rat Angiopoietin-1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Angiopoietin-1 has been precoated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Angiopoietin-1 amount of sample captured in plate.

  • Notes

    Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-rat Angiopoietin-1 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- rat Angiopoietin-1 antibody 1 x 130µl
    Lyophilized recombinant rat Angiopoietin-1 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • AGP 1
    • AGP1
    • AGPT
    • ANG
    • ANG 1
    • ANG-1
    • ANG1
    • Angiopoietin-1
    • Angiopoietin1
    • ANGP1_HUMAN
    • ANGPT 1
    • Angpt1
    • KIAA0003
    • OTTHUMP00000227592
    • OTTHUMP00000227599
    • OTTHUMP00000227600
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Rat Angiopoietin-1 ELISA Kit (ab213920) Standard Curve.

Protocols

References

ab213920 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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