Overview

  • Product name

    Rat ANP ELISA Kit (NPPA)
    See all Natriuretic peptides A kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Tissue
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.2 ng/ml
  • Range

    0.25 ng/ml - 4 ng/ml
  • Assay time

    5h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Pig
  • Product overview

    Abcam’s ANP (NPPA) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ANP concentrations in cell culture supernatants, tissue-extract, serum and plasma.


    An ANP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an ANP specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of ANP captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Rat ANP measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab108797.

Protocols

References

This product has been referenced in:

  • Chhabra A  et al. Glucose-6-phosphate dehydrogenase is critical for suppression of cardiac hypertrophy by H2S. Cell Death Discov 4:6 (2018). Read more (PubMed: 29531803) »
  • Nehra S  et al. Nanocurcumin-pyrroloquinoline formulation prevents hypertrophy-induced pathological damage by relieving mitochondrial stress in cardiomyocytes under hypoxic conditions. Exp Mol Med 49:e404 (2017). Read more (PubMed: 29611541) »
See all 5 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Good product!

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The ELISA Kit is easy to use and work well.
Rat plasma was used with five times dilution.(For reference)

Miss. Rui Ma

Verified customer

Submitted Jun 20 2017

Answer

We tested mouse samples only at two different concentrations, which is not ideal to verify linearity. We did get a significant signal, but there is not a satisfying reduction in signal at dilution 1/10 vs the neat sample. The rat values titrate out better. The data in the image on our datasheet is from undiluted mouse sample, as this is the dilution that I report the human and rat values.

We have therefore got a significant signal in mouse, but the linearity has not been fully assessed. As the standard curve is using Rat ANP, the accurate measurements for other species must be considered possibly skewed.

We do not guarantee these species, as the test has only been performed once with a limited number of dilutions, hence the linearity could not accurately be assessed (human is better than mouse at dilutions 1-1/10).

However, if you would like to test this kit in mouse and send us your results, we could offer you our testing program.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Purchase the product at regular price and forward your order reference number or PO back to me.

2. I will generate a personal discount code for you valued at the purchase price of the product.

3. Submit your results including an image via our online Abreview system, including your discount code in the additional notes section of the Abreview.

4. Once the Abreview is submitted, the discount code will become active.

5. Apply your discount code on your next order to receive that value off. The purchase must be made within 6 months of the code being issued. The entire value of the discount code must be applied at once and codes will not keep balances.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

Read More

Answer



The lab has testd several plasma and serum samples, all at no dilution (1x). The value may be 0.3 ng/ml for human plasma/serum, but it may be higher or lower depending on the sample. Each sample is different and some optimization may be required.

Read More

Answer



Human plasma and serum has been tested with this kit. Rat EDTA plasma has also been tested, so human EDTA plasma should work with the kit as well.

Read More

Answer

Thank you for your enquiry regarding ab108797 and ab108815. I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

It is hard to answer the question without any detail information. Could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

We are particularly interested in the followings:

How the accuracy has been determined? By CV? By standard deviation?
Have any controls have used? If you use cv %, are you using OD or concentration?



Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

Read More

Answer

Thank you for your patience with this reply.
We would recommend doing a serial dilution to see which protein concentration will fall within the standard curve. Take a look at our sample preparation guide for additional information. https://www.abcam.com/index.html?pageconfig=resource&rid=14666
I hope this information helps. Please contact us with any other questions.

Read More

Question
Answer

The amount of ANP in the vial of standard for the lot you have is 20 nanograms. Please contact us if you have other questions about the assay.

Read More

Answer

Thank you very much for your inquiry. I am very sorry for the delay.

We tried to find a concrete value to propose for the extraction kit, however this is not possible.We recommend that each laboratory does optimize the conditions, dilutions factor and extraction protocol according to the set up and the samples. It really depends on the samples and the research project. In general, our expertwould say if you can not get anOD valuefor your samples in the middle of the standard curve (around 0.5 ng/ml) than she would recommend todo the extraction protocol. However asthe level of ANP in healthy individuals is usually very low,most of the time you would perform then the extraction.I hope this information is nevertheless helpful.

In regards to the expiry date, we do not have an official expiry date for our products. I can confirm howeverthat we do guarantee all our products for at least 6 months after receipt. Please store them as indicated on the datasheet. For more information about our guarantee, please visit also https://www.abcam.com/abpromise

I wish you good success with your research. Please do not hesitate to contact us again, should you have any other question.

Read More

Question
Answer

Thank you for your enquiry. I am sorry it has taken some time to confirm this information for you.

As discussed on the telephone, I am sorry that asfar as we are aware, ab108797ANP Rat ELISA kithas never been tested with samples from horse. All tested species cross-reactivity information is stated on our datasheets, and these are updated as soon as any new information is brought to our attention. I am sorry that we also have no data from testing on saliva samples.

I have checked the alignment of the immunogen with the horse sequence. This has 96% alignment and so the antibodies in this kit are theoretically likely to detect the horse ANP protien. However, please note that we are not able to provide a guarantee for this species as it has not been tested.

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

Read More

Answer

Thank you for contacting Abcam regarding this issue. It is okay to use heparin coated tubes and avoid having to use the 0.1M sodium citrate. I hope this helps. If there is anything else that I can help you with please let me know.

Read More

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up