Overview

  • Product name

  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell Lysate, Tissue Lysate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    15 pg/ml
  • Range

    15 pg/ml - 15000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell Lysate = 98.06 69% - 108%
    Tissue Lysate = 99.34 70% - 121%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s beta NGF Rat ELISA Kit (ab193736) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat beta NGF in cell lysate and tissue lysate.

    This assay employs an antibody specific for rat beta NGF coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures beta NGF present in the samples. The wells are washed and biotinylated anti-rat beta NGF antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of beta NGF bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    2X Cell Lysis Buffer 1 x 5ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    5X Sample Diluent Buffer 1 x 10ml
    Biotinylated rat beta-NGF detection antibody (lyophilized) 2 vials
    Pre-coated rat beta-NGF Microplate (12 strips x 8 wells) 1 unit
    rat beta-NGF Standards (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.
  • Cellular localization

    Secreted.
  • Alternative names

    • Beta nerve growth factor
    • Beta NGF
    • HSAN5
    • MGC161426
    • MGC161428
    • Nerve growth factor (beta polypeptide)
    • Nerve growth factor beta polypeptide
    • Nerve growth factor beta subunit
    • NGFB
    • NID67
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab193736

Protocols

References

This product has been referenced in:

  • Piao JM  et al. MicroRNA-381 Favors Repair of Nerve Injury Through Regulation of the SDF-1/CXCR4 Signaling Pathway via LRRC4 in Acute Cerebral Ischemia after Cerebral Lymphatic Blockage. Cell Physiol Biochem 46:890-906 (2018). Read more (PubMed: 29669322) »
  • Ohta M  et al. IL-1ß and TNF-a suppress TGF-ß-promoted NGF expression in periodontal ligament-derived fibroblasts through inactivation of TGF-ß-induced Smad2/3- and p38 MAPK-mediated signals. Int J Mol Med 42:1484-1494 (2018). Read more (PubMed: 29901090) »
See all 3 Publications for this product

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