• Product name
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 40 pg/ml
  • Range
    0.049 ng/ml - 100 ng/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 132.1 123% - 142%
    Serum 111.7 102% - 121%
    Plasma 97.02 70% - 126%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s betaNGF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat betaNGF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Rat betaNGF coated on a 96-well plate. Standards and samples are pipetted into the wells and betaNGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat betaNGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of betaNGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    betaNGF Microplate (12 strips x 8 wells) 1 unit
    betaNGF Standard Rat (recombinant) (lyophilized) 2 vials
    Biotinylated anti-rat beta-NGF (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.
  • Cellular localization
  • Alternative names
    • Beta nerve growth factor
    • Beta NGF
    • HSAN5
    • MGC161426
    • MGC161428
    • Nerve growth factor (beta polypeptide)
    • Nerve growth factor beta polypeptide
    • Nerve growth factor beta subunit
    • NGFB
    • NID67
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab100757.

  • Representative Standard Curve using ab100757.



This product has been referenced in:
  • Jiang YH  et al. Cardiac Dysregulation and Myocardial Injury in a 6-Hydroxydopamine-Induced Rat Model of Sympathetic Denervation. PLoS One 10:e0133971 (2015). Read more (PubMed: 26230083) »
  • Liu J  et al. Contribution of nerve growth factor to upregulation of P2X3 expression in DRG neurons of rats with femoral artery occlusion. Am J Physiol Heart Circ Physiol 301:H1070-9 (2011). Read more (PubMed: 21642505) »

See all 2 Publications for this product

Customer reviews and Q&As

The antibodies in this kit were raised with the mature form of the protein bNGF (aa 122-241). Based on sequence similarities the pro-NGF could possibly be recognized. However, we have not tested the pro-form with this kit so I am afraid we ca...

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Thank you for contacting us. The minimum detectable dose of beta-NGF is typically less than 15 pg/ml with this kit. We present this range with the more conservative low point of 49pg/ml to ensure accurate data. Regarding standards, the included stan...

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Thank you for your enquiry.

Regarding question 1: Yes you are correct. The initial concentration of the resuspended standard will be 50ng/ml.

Regading the region of detectable concentration: The minimum detectable dose of NGF is typ...

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Thank you for alerting us to this problem.

We will be sending you 2 vials of item F and 1 vial of item G.

I am sorry about the mix-up, but I hope we can get this squared away for you. Please let me know if you have any other ...

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Thank you for contacting Abcam. I have had a reposnse from the lab about the issues you were having with ab100757. Please see their response below: Were the wells with precipitate also “dark blue” before adding the Stop Solution? If so, thi...

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Thank you for contacting Abcam. I just wanted to let you know that I am still waiting to hear back from the lab about the problems you have been having with ab100757 and I will contact you again, as soon as I hear back from them. I do appreciate your p...

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Thank you for your inquiry.  While ab100757 has not been validated with urine (only serum, plasma, and cell culture supernatants), our ELISA kits are very accommodating to many different types of samples so urine along with other body fluids will likel...

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While this kit has only been validated with serum, plasma, and cell culture supernatants, it is very accommodating and should work with most sample types including cell and tissue lysates. Attached are some general tips to help the customer prepa...

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