• Product name
    Rat BNP 32 ELISA Kit
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall 4.7%
    Sample n Mean SD CV%
    Overall 9.4%
  • Sample type
    Cell culture supernatant, Serum, Plasma, Tissue
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 0.06 ng/ml
  • Range
    0.031 ng/ml - 2 ng/ml
  • Assay time
    5h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s BNP 32 Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BNP 32 in plasma, serum, tissue extract, and cell culture supernatants.

    A BNP 32 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a BNP 32 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of BNP 32 captured in plate.

  • Notes

    There is significant cross-reactivity between BNP32 and BNP45.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Rat BNP 32 Antibody 1 x 120µl
    BNP 32 Microplate (12 x 8 well strips) 1 unit
    BNP 32 Standard 1 vial
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • Tissue specificity
    Brain and also in atria, but at much lower levels than ANP.
  • Sequence similarities
    Belongs to the natriuretic peptide family.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • BNP(1-32)
    • BNP(5-29)
    • BNP-32
    • Gamma-brain natriuretic peptide
    • nppb
    see all


Our Abpromise guarantee covers the use of ab108815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.


  • Representative Standard Curve using ab108815.



This product has been referenced in:
  • Du Y  et al. Altered expression of PPAR-? and TRPC in neonatal rats with persistent pulmonary hypertension. Mol Med Rep 16:1117-1124 (2017). Read more (PubMed: 28627661) »
  • Kumar V  et al. Amalaki rasayana, a traditional Indian drug enhances cardiac mitochondrial and contractile functions and improves cardiac function in rats with hypertrophy. Sci Rep 7:8588 (2017). Sandwich ELISA . Read more (PubMed: 28819266) »

See all 3 Publications for this product

Customer reviews and Q&As

Le laboratoire m’a confirmé que vous pouvez utiliser un tampon EDTA plutôt qu’un tampon de citrate pour vos échantillons de plasma.

Merci de nous avoir contactés.

Les collaborateurs du laboratoire où les kits ELISA ab108815 et ab108816 ont été développés m'ont informé que:

1) Les deux kits peuvent en effet êtr...

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Thank you for your enquiry regarding ab108797 and ab108815. I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

It is hard to answer the question without any detail information. Cou...

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Thank you for your enquiry.

I can confirm that the first difference between these kits is that ab108815 detects the 32 amino acid form of BNP, andab108816 detects the larger 45 amino acid form of the protein.

According to a litera...

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