Overview

  • Product name

    Rat BNP 45 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Sample type

    Cell culture supernatant, Serum, Plasma, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.03 ng/ml
  • Range

    0.031 ng/ml - 1 ng/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s BNP 45 rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BNP 45 in plasma, serum, tissue extract, and cell culture supernatants.


    A BNP 45 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a BNP 45 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of BNP 45 captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Notes

    There is significant cross-reactivity between BNP32 and BNP45.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    70X Biotinylated Rat BNP 45 Antibody 1 x 90µl
    BNP 45 Microplate (12 x 8 well strips) 1 unit
    BNP 45 Standard 1 vial
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • Tissue specificity

    Brain and also in atria, but at much lower levels than ANP.
  • Sequence similarities

    Belongs to the natriuretic peptide family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • ANFB_HUMAN
    • BNP(1-32)
    • BNP(5-29)
    • BNP-32
    • BNP-45
    • Gamma-brain natriuretic peptide
    • nppb
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Standard curve : mean of duplicates (+/-SD) with background readings subtracted

  • Rat BNP45 measured in biological fluids showing quantity (pg) per mL of tested sample

Protocols

References

This product has been referenced in:

  • Eid RA  et al. Fas/FasL-mediated cell death in rat's diabetic hearts involves activation of calcineurin/NFAT4 and is potentiated by a high-fat diet rich in corn oil. J Nutr Biochem 68:79-90 (2019). Read more (PubMed: 31030170) »
  • Hua Y  et al. Alda-1, an aldehyde dehydrogenase-2 agonist, improves long-term survival in rats with chronic heart failure following myocardial infarction. Mol Med Rep 18:3159-3166 (2018). Read more (PubMed: 30066916) »
See all 8 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Answer

Thank you for your enquiry.

Currently, this is the only BNP 45 ELISA kit (ab108816) which recognizes rat BNP 45. We sell primary antibodies which detect human BNP:

ab20984,

ab19645,

ab19646,

ab47699,

ab66240

I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

Rat BNP 45 ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews
Rat BNP 45 ELISA Kit
The ELISA Kit is easy to use and works well.
Rat plasma was used with 10 times dilution.(For reference)
Antibody incubation time is a bit long.

Abcam user community

Verified customer

Submitted Jul 10 2017

Question
Answer

Our samples showed around 40% cross-reactivity for rabbit protein.

Read More

Answer

Merci de nous avoir contactés.

Les collaborateurs du laboratoire où les kits ELISA ab108815 et ab108816 ont été développés m'ont informé que:

1) Les deux kits peuvent en effet être utilisés sur plasma hépariné.

2) BNP32 et BNP45 correspondent à 2 formes de la BNP. Le croisement entre les 2 kits est d'environ 60%. D'après mes collaborateurs, il est parfois décrit que la BNP45 est plus abondante que la BNP32, et parfois il est décrit que c'est l'inverse. Le choix sur la forme de la BNP à mesurer est donc laissé à l'expérimentateur.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Read More

Question
Answer

Vielen Dank für Ihren Anruf.

Wenn das Futter keinen Einfluss auf die Produktion dieser Peptide hat (wie durch zB salzreiche Nahrung), sehe ich keinen Grund, warum die Ratten fasten sollten.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

The best way to test the viability is using the kit. The kits should be fine at -20C so please use the kit without any hesitation.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
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Question


The answers followed below:
1) The sample collected to quantify the concentration of BNP was the
blood plasma of Wistar rats.
After the samples
had been collected it was transferred to a test tube suitable for the
volume collected and
centrifuged for 10 min at 2000 rcf and 4C. After centrifugation,
plasma was divided
into 4 aliquots of 200ul in ependorfs of 1.5 mL. After having been
aliquoted plasma was
frozen in liquid nitrogen and stored in a freezer at-80C until the day
that one of these
aliquots were unfreezed to perform the ELISA.

2) In this study two methods are being used to quantify the BNP: ELISA
and qRT-PCR.
The quantification is being processed in two distinct periods, two and
five months after
the animals have completed treatment to which they have undergone. The
results of the
first ELISA were consistent with the results of qRT-PCR, meaning that
the average of
the analyzed groups had the same place in both methods, for example:
group A had the
second highest average, the group the higher average B and group C at
the third highest
average, all in both the ELISA and the qRT-PCR. But this did not happen with the
second kit. In the second ELISA, the A group obtained the highest
average in the first
ELISA, but the second highest average in qRT-PCR and group B had the
second highest
average ELISA, but the highest average qRT-PCR, and group C was
remained the third
highest average in both the ELISA and the qRT-PCR. But it is not only
this, compared
to the first kit, the second kit had twice the samples with 15% CV
above (the first of the
samples was 6.06% with CVs higher than 15% and the second 12%). The
calculation of
intra-assay reproducibility of the first was 4.2% and 6.6% of the
second. As all samples
were collected under the same conditions, the first and second kit
being from different
batches and the second kit submitting the fault of does not have the
information of the
quantity of pattern provided, although not having problems with the
standard curve,
these facts lead me to suspect that may have occurred other problems
in the production
of the kit beyond of not contain the addition amount of the standard provided.

3) To perform both kits, all the step by step information of the same
datasheet were followed. None of the kits used diluted samples.

Read More
Answer

Thank you for your reply and the additional information.

We have the following PO on file: xxx.The order was from xxx and the lot number matches what you had sent, thus I think this is the correct order.


I have now arranged the free of charge replacement.

Please accept my sincere apologies for the problems with the second kit.
Please do not hesitate to contact me if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

I am very sorry to hear about the problems your customer had to face because of the second kit. I sincerely apologize about that! Yes, I'd be happy to arrange for a free of charge replacement.


Could you please let me know your order number or PO number with us for the second kit?

Also, for our internal record, I would like to receive a couple more details from the customer:
1) What type of samples were used and how were they prepared?
2) What do the data look like? In which way were the data incorrect?
3) Was the protocol followed step by step or were any deviations included?


I look forward to hear back from you and to assist you in resolving this case.

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Answer

Gracias por contactarnos y alertarnos de este fallo.



Lamento mucho que el vial de estándar del nuevo lote enviado no indicara ningún dato acerca de la cantidad contenida. Sin duda se trata de un error que estamos tratando de resolver lo antes posible para que las nuevas etiquetas, así como la datasheet contengan la información necesaria.

He comprobado que el vial recibido del lote XXXX contiene la misma cantidad de estándar que el anterior (4ng).

También aprovecho para recordarte que todos nuestros productos están cubiertos por la garantía Abpromise de 6 meses, y en caso de no funcionar como se especifica en su datasheet, se os reemplazará el producto de forma gratuita o se reembolsará el importe del mismo.

Espero haberte ayudado. Para cualquier otra consulta o sugerencia, por favor no dudes en volvernos a contactar.

Read More

Answer

Thank you for your enquiry and your interest.

As the Protocol booklet indicates we would suggest adding 50 μl of Chromogen Substrate per well and incubate for about 15 minutes or till the optimal blue colour density develops. The optimal time points and the dilution(s) needs to be optimized by the end user since the BNP 45 levels depends on the samples.

I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

1-10 of 11 Abreviews or Q&A

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