Rat brain normal tissue lysate - membrane extract (ab29473)

Overview

  • Product name
    Rat brain normal tissue lysate - membrane extract
  • Description
    Rat brain membrane extract lysate
  • General notes


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage buffer
    Constituents: 1% Tergitol-NP40, Potassium chloride, 0.1% Bromophenol blue, Magnesium chloride, 1.2% Beta mercaptoethanol, 2% SDS, HEPES, Sucrose, 10% Glycerol

    With Protease inhibitor cocktail/EDTA/Sodium deoxycholate
  • Concentration information loading...
  • Lysate notes
    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab29473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

References

ab29473 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry. Yes, boiling is recommended before loading the sample into gel. Please do not hesitate to contact us if you have additional questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up