Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
Our Abpromise guarantee covers the use of ab29473 in the following tested applications.
|WB||Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.|
ab29473 has not yet been referenced specifically in any publications.