Overview

  • Product name

    Rat CD44 ELISA Kit
    See all CD44 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1.76ng/ml 0.09 5.1%
    2 16 5.5ng/ml 0.35 6.4%
    3 16 12.8ng/ml 0.73 5.7%
    Inter-assay
    Sample n Mean SD CV%
    1 24 2.1ng/ml 0.16 7.8%
    2 24 7.6ng/ml 0.52 6.9%
    3 24 14ng/ml 0.88 6.3%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    312 pg/ml - 20000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    The Rat CD44 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213928) is designed for the quantitative measurement of Rat CD44 in cell culture supernatants, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD44 has been precoated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for CD44 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat CD44 amount of sample captured in plate.

  • Notes

    CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-rat CD44 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- rat CD44 antibody 1 x 130µl
    Lyophilized recombinant rat CD44 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • Tissue specificity

    Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • Sequence similarities

    Contains 1 Link domain.
  • Domain

    The lectin-like LINK domain is responsible for hyaluronan binding.
  • Post-translational
    modifications

    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • LHR
    • BA-1
    • CD 44
    • CD44
    • CD44 antigen
    • CD44 molecule
    • CD44 molecule (Indian blood group)
    • CD44_HUMAN
    • CDw44
    • CDW44 antigen
    • Cell surface glycoprotein CD44
    • chondroitin sulfate proteoglycan 8
    • CSPG8
    • ECMR-III
    • Epican
    • Extracellular matrix receptor III
    • GP90 lymphocyte homing/adhesion receptor
    • HCELL
    • hematopoietic cell E- and L-selectin ligand
    • Heparan sulfate proteoglycan
    • Hermes antigen
    • homing function and Indian blood group system
    • HSA
    • HUTCH-I
    • HUTCH1
    • HUTCHI
    • Hyaluronate receptor
    • IN
    • INLU-related p80 Glycoprotein
    • MC56
    • MDU2
    • MDU3
    • MGC10468
    • MIC4
    • MUTCH I
    • MUTCH1
    • PGP-1
    • PGP-I
    • PGP1
    • Phagocytic glycoprotein 1
    • Phagocytic glycoprotein I
    • Soluble CD44
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Rat CD44 ELISA Kit (ab213928) Standard Curve.

Protocols

References

ab213928 has not yet been referenced specifically in any publications.

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