Rat CD62L ELISA Kit (Selectin L) (ab100776)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat CD62L ELISA Kit (Selectin L)
See all CD62L kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 6 pg/ml -
Range
8.23 pg/ml - 6000 pg/ml -
Recovery
96 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 98.42 89% - 107% Serum 97.06 86% - 107% Plasma 94.33 85% - 103% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s CD62L Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CD62L in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for CD62L coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62L present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CD62L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62L bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti Rat CD62L 2 vials CD62L Microplate (12 x 8 wells) 1 unit Recombinant rat CD62L Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
CD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. -
Cellular localization
Cell Membrane; single-pass type I membrane protein. -
Alternative names
- A.11
- AI528707
- CD62 antigen ligand
see all -
Database links
- Entrez Gene: 29259 Rat
- SwissProt: Q63762 Rat
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab100776 has not yet been referenced specifically in any publications.