Overview

  • Product name

    Rat CD62L ELISA Kit (Selectin L)
    See all CD62L kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 6 pg/ml
  • Range

    8.23 pg/ml - 6000 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.42 89% - 107%
    Serum 97.06 86% - 107%
    Plasma 94.33 85% - 103%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s CD62L Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CD62L in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for CD62L coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62L present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CD62L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62L bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti Rat CD62L 2 vials
    CD62L Microplate (12 x 8 wells) 1 unit
    Recombinant rat CD62L Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    CD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.
  • Cellular localization

    Cell Membrane; single-pass type I membrane protein.
  • Alternative names

    • A.11
    • AI528707
    • CD62 antigen ligand
    • CD62 antigen-like family member L
    • CD62L antigen
    • gp90 MEL
    • hLHRc
    • IgA nephropathy, susceptibility to, included
    • L Selectin
    • L selectin precursor
    • LAM 1
    • LAM1
    • LECAM1
    • Leu 8
    • Leu8
    • Leukocyte adhesion molecule 1
    • Leukocyte endothelial cell adhesion molecule 1
    • Leukocyte surface antigen Leu 8
    • Leukocyte surface antigen Leu8
    • Leukocyte-endothelial cell adhesion molecule 1
    • LNHR
    • LSEL
    • Ly-22
    • Ly-m22
    • Lyam 1
    • LYAM1
    • Lymph node homing receptor
    • Lymphocyte adhesion molecule 1
    • Lymphocyte antigen 22
    • Lymphocyte surface MEL-14 antigen
    • PLNHR
    • Selectin L
    • Selectin L (lymphocyte adhesion molecule 1)
    • Selectin, lymphocyte
    • SELL
    • TQ1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100776

  • Representative standard curve using ab100776

Protocols

References

ab100776 has not yet been referenced specifically in any publications.

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