Overview

  • Product name

    Rat Ceruloplasmin ELISA Kit (CERP)
    See all Ceruloplasmin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.4%
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Cell Lysate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.3 ng/ml
  • Range

    0.31 ng/ml - 20 ng/ml
  • Recovery

    96 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s Ceruloplasmin (CERP) rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ceruloplasmin in urine and cell culture supernatants.


    A Ceruloplasmin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ceruloplasmin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ceruloplasmin captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab108819.

Protocols

References

ab108819 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry and interest in our products.

Your customer can use the kit (ab108819) to determine cell lysate ceruloplasmin level, though it is advised to perform a serial dilution of the sample to determine what is the most optimal dilution.

If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

Thank you for your enquiry.

This kit has been tested on cell culture supernatant and urine as samples - but not on serum.

I would advise you to take a look at the datasheet and the Protocol Booklet for further information.

https://www.abcam.com/ab108819

https://www.abcam.com/ps/products/108/ab108819/documents/ab108819%20Ceruloplasmin%20Rat%20ELISA_v2%20(Website).pdf

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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