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|Sample type||Average %||Range|
|Cell culture supernatant||112.5||99% - 122%|
|Plasma||107.9||97% - 125%|
Abcam’s CINC-1 (CXCL-1) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of rat CINC-1 in serum, plasma and cell culture supernatants. (Collect plasma using EDTA and heparin as an anticoagulant). Citrate is not recommended as anticoagulants for use in this assay. Rat CINC-1 concentration in normal plasma is very low and it may not be detected in this assay.
This assay employs an antibody specific for rat CINC-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CINC-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat CINC-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CINC-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Rat CINC-1 in normal serum with ab155462, only in serum spiked with Rat CINC-1.
Sensitivity of the assay is dependant on the Assay Diluent used. Please refer to Protocol Booklet for more details.
|Components||1 x 96 tests|
|200X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|5X Assay Diluent D||1 x 15ml|
|Biotinylated anti-rat CINC-1||2 vials|
|CINC-1 Microplate (12 x 8 wells)||1 unit|
|Recombinant Rat CINC-1 Standard (lyophilized)||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab155462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab155462 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"