Overview

  • Product name
    Rat CINC-3 ELISA Kit (CXCL2)
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    7.4 pg/ml - 1800 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Plasma 91.87 72% - 111%
    Cell culture media 83.48 75% - 89%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam's CINC-3 (CXCL2) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of rat CINC-3 (CXCL2) in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for CINC-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and CINC-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CINC-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CINC-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab155463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155463 - Assay Diluent B.

  • Representative standard curve using ab155463 - Assay Diluent C.

Protocols

References

This product has been referenced in:
  • Mazzio EA  et al. Natural product HTP screening for attenuation of cytokine-induced neutrophil chemo attractants (CINCs) and NO2- in LPS/IFN? activated glioma cells. J Neuroimmunol 302:10-19 (2017). Read more (PubMed: 27956075) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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