Key features and details
- Sensitivity: 2 pg/ml
- Range: 7.4 pg/ml - 1800 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Product nameRat CINC-3 ELISA Kit (CXCL2)
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 2 pg/ml
Range7.4 pg/ml - 1800 pg/ml
Sample specific recovery Sample type Average % Range Plasma 91.87 72% - 111% Cell culture media 83.48 75% - 89%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Rat
Abcam's CINC-3 (CXCL2) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of rat CINC-3 (CXCL2) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for CINC-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and CINC-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CINC-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CINC-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-rat CINC-3 2 vials CINC-3 Microplate (12 x 8 wells) 1 unit Recombinant Rat CINC-3 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
- C-X-C motif chemokine 2
- CINC 3
ab155463 has been referenced in 1 publication.
- Mazzio EA et al. Natural product HTP screening for attenuation of cytokine-induced neutrophil chemo attractants (CINCs) and NO2- in LPS/IFN? activated glioma cells. J Neuroimmunol 302:10-19 (2017). PubMed: 27956075