• Product name

    Rat CNTF ELISA Kit
    See all CNTF kits
  • Detection method

  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    8.23 pg/ml - 2000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.3 89% - 108%
    Serum 105.6 93% - 112%
    Plasma 104.2 92% - 110%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s CNTF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat CNTF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for rat CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-rat CNTF 2 vials
    CNTF Microplate (12 strips x 8 wells) 1 unit
    Recombinant rat CNTF Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury. In addition to its neuronal actions, CNTF also acts on non neuronal cells such as glia, hepatocytes, skeletal muscle, embryonic stem cells and bone marrow stromal cells.
  • Cellular localization

  • Alternative names

    • AI429687
    • Ciliary Neurotrophic Factor
    • HCNTF
    • MGC41235
    • OTTHUMP00000174731
    • OTTMUSP00000029848
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100758

  • Representative standard curve using ab100758



ab100758 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A


Epitope mapping has not been performed so the exact binding sequence that the antibody pair recognizes is not known. All that is known is that the antibodies are binding somewhere on the full length protein (http://www.uniprot.org/uniprot/P20294).

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Abreview for CNTF Rat ELISA Kit

Average Average 3/5 (Ease of Use)

Sample: Mouse Serum (blood)
Specification: blood
Amount of Sample: 100 µg
Type of Kit Used: ELISA
Positive control: rat serum
purified CNTF
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Wanted to use the kit to measure CNTF in mouse serum, but it did not work for the mouse samples. Mouse serum was used in multiple amounts, but the plate wells containing the mouse samples were no different than background. The kit worked well with serial dilutions of my positive control (rat serum), though. The kit is otherwise easy to use.

Abcam user community

Verified customer

Submitted Dec 21 2011


Thank you for your inquiry.  Unfortunately, we have not tested mouse samples with the ab99994 kit or ab100758 kit. The lab didn't think that the human kit ab99994 would detect mouse samples since it didn't work in rat (the mouse and rat proteins are 95% homologous). Considering all this, there is a chance that the rat kit ab100758 may recognize the mouse protein, since the proteins are so similar.  If you would like to test ab100758 in mouse, let me know and we can set you up with a testing discount.  If you purchase the kit, test it in mouse, and it does or does not work, we would reward you with a free primary antibody or that price off another ELISA kit after you submit an Abreview to us with your data.  Please let me know if you would like to test ab100758 in mouse and I will set you up in our system before you purchase it.  I look forward to your reply.    

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Thank you for your email.  I have addressed your question below. ab100758 : No cross reactivity data is available testing mouse samples with this rat kit therefore the only way to know would be to test and see.  However, both the capture and detection antibody are polyclonal, and since rat and mouse CNTF are 95% identical at the amino acid level, it is quite likely this kit will work with mouse samples. If you are interested in testing this kit in mouse samples, please see below on how to get a free antibody or free kit for testing this kit with your mouse samples: =================== Thank you very much for your interest in ab100758: To our knowledge, ab100758 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab100758 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free kit OR 1 free antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab100758 in mouse. I will then send a discount code. This code must be issued before purchasing ab(XXX) so please wait for my reply before ordering. 2. Purchase ab100758 either by phone, fax, or online (www.abcam.com). 3. Test it in mouse. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for 1 free kit OR 1 free antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab100758 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.      

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I am very pleased to hear you would like to accept our offer and test ab100758 in mouse. This code will give you 1 free kit OR 1 free antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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