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Immunology Innate Immunity Complement Classical Pathway
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Rat Complement C3 ELISA Kit (ab157731)

  • Datasheet
  • SDS
  • Protocol Booklet
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Standard Curve

    Key features and details

    • Sensitivity: 2.82 ng/ml
    • Range: 12.5 ng/ml - 800 ng/ml
    • Sample type: Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Rat

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    Overview

    • Product name

      Rat Complement C3 ELISA Kit
      See all C3 kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 10%
    • Sample type

      Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      2.82 ng/ml
    • Range

      12.5 ng/ml - 800 ng/ml
    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum > 85 % - %
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Rat
    • Product overview

      Abcam's Complement C3 Rat ELISA Kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for measuring Complement C3 in rat serum and plasma samples.

      In this assay the Complement C3 present in samples reacts with the anti-Complement C3 antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Complement C3 antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Complement C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Complement C3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Complement C3 in the test sample. The quantity of Complement C3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X HRP-conjugated anti-Rat Complement C3 antibody 1 x 150µl
      20X Wash Buffer Concentrate 1 x 50ml
      5X Diluent Concentrate 1 x 50ml
      Chromogen Substrate Solution 1 x 12ml
      Rat Complement C3 Calibrator (lyophilized) 1 vial
      Rat Complement C3 ELISA Microplate 1 unit
      Stop Solution 1 x 12ml
    • Research areas

      • Immunology
      • Innate Immunity
      • Complement
      • Classical Pathway
      • Immunology
      • Innate Immunity
      • Complement
      • Alternative Pathway
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Complement ELISA kits
    • Function

      C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
      Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    • Tissue specificity

      Plasma.
    • Involvement in disease

      Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
      Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
      Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
    • Sequence similarities

      Contains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Post-translational
      modifications

      C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P01024 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Acylation stimulating protein cleavage product
      • AHUS5
      • ARMD9
      • ASP
      • C3
      • C3 and PZP like alpha 2 macroglobulin domain containing protein 1
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
      • c3 complement
      • C3adesArg
      • CO3_HUMAN
      • Complement C3
      • Complement C3 alpha chain
      • Complement C3b alpha' chain
      • Complement C3c alpha' chain fragment 1
      • Complement C3c alpha' chain fragment 2
      • Complement C3c alpha'' chain fragment 2
      • Complement C3d fragment
      • Complement C3dg fragment
      • Complement C3f fragment
      • Complement C3g fragment
      • Complement component 3
      • Complement factor 3
      • CPAMD1
      • HEL S 62p
      • omplement C3 beta chain
      see all
    • Database links

      • Entrez Gene: 24232 Rat
      • SwissProt: P01026 Rat
      • Unigene: 11378 Rat

      Associated products

        Images

        • Standard Curve
          Standard Curve
          Representative standard curve using ab157731 Complement C3 Rat ELISA Kit.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (3)

        Publishing research using ab157731? Please let us know so that we can cite the reference in this datasheet.

        ab157731 has been referenced in 3 publications.

        • Yang Z  et al. Early Complement and Fibrinolytic Activation in a Rat Model of Blast-Induced Multi-Organ Damage. Mil Med 184:282-290 (2019). PubMed: 30901474
        • Hata J  et al. Complement activation by autoantigen recognition in the growth process of benign prostatic hyperplasia. Sci Rep 9:20357 (2019). PubMed: 31889151
        • Haihua C  et al. Cobra Venom Factor-induced complement depletion protects against lung ischemia reperfusion injury through alleviating blood-air barrier damage. Sci Rep 8:10346 (2018). PubMed: 29985461

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