Overview

  • Product name

    Rat CXCL1 ELISA Kit
    See all GRO alpha kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Rat serum 8 10.1%
    Inter-assay
    Sample n Mean SD CV%
    Rat serum 3 10%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.61 pg/ml
  • Range

    9.38 pg/ml - 600 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 99 88% - 109%
    Cell culture extracts 103 100% - 104%
    Cell culture media 95 90% - 102%
    Tissue Culture Media 96 85% - 116%
    Heparin Plasma 114 110% - 118%
    EDTA Plasma 106 103% - 108%
    Citrate Plasma 97 88% - 112%

  • Assay time

    1h 45m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
    Does not react with: Cow, Human
  • Product overview

    CXCL1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rat CXCL1 protein in serum, plasma, cell culture supernatant, and cell extract samples.


    The ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples in Sample Diluent 10BS: 2.13 pg/mL
    Samples in 1X Cell Extraction Buffer PTR: 1.61 pg/mL

  • Notes

    CXCL1 (previously called GRO1 oncogene) is a member of the CXC family of chemokines. CXCL1 expression is reported in lymphocytes such as macrophages and neutrophils as well as epithelial cells. It plays a role in neutrophil chemotaxis. CXCL1 is involved in spinal cord development via inhibition of oligodendrocyte precursor migration as well angiogenesis, arteriogenesis, inflammation, wound healing and tumorigenesis.

    Rat CXCL2 and CXCL3 share 65% and 64% sequence homology with rat CXCL1, respectively. Additionally, mouse CXCL1 is 89% homologous with rat CXCL1.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT 1 x 30ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI 2 x 6ml
    Plate Seals 1 unit
    10X Rat CXCL1 Capture Antibody 1 x 600µl
    10X Rat CXCL1 Detector Antibody 1 x 600µl
    Rat CXCL1 Lyophilized Recombinant Protein 2 vials
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C-X-C motif chemokine 1
    • chemokine (C-X-C motif) ligand 1
    • Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
    • CINC-1
    • CXCL1
    • Cytokine-induced neutrophil chemoattractant 1
    • Fibroblast secretory protein
    • Fsp
    • Gro
    • Gro 1
    • Gro A
    • GRO protein, alpha
    • GRO-alpha(1-73)
    • GRO-alpha(6-73)
    • Gro1
    • Gro1 oncogene
    • GRO1 oncogene (melanoma growth stimulating activity, alpha)
    • GRO1 oncogene (melanoma growth-stimulating activity)
    • GROa
    • GROA_HUMAN
    • Growth-regulated alpha protein
    • KC
    • KC chemokine, mouse, homolog of
    • Melanoma growth stimulatory activity
    • melanoma growth stimulatory activity alpha
    • Melanoma growth stimulatory activity, alpha
    • MGSA
    • MGSA alpha
    • MGSA-a
    • N51
    • NAP-3
    • NAP3
    • Neutrophil-activating protein 3
    • Platelet-derived growth factor-inducible protein KC
    • Scyb 1
    • Scyb1
    • Secretory protein N51
    • Small inducible cytokine subfamily B, member 1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab219044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Standard curve comparison between rat CXCL1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

  • The concentrations of CXCL1 were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 930.66 pg/mL in neat serum, 756.22 pg/mL in neat plasma (citrate), 674.04 pg/mL in neat plasma (EDTA), and 891.86 pg/mL in neat plasma (heparin).

  • The concentrations of CXCL1 were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 245.16 pg/mL in neat serum, 75.79 pg/mL in neat plasma (citrate), 24.53 pg/mL in neat plasma (EDTA), and 137.19 pg/mL in neat plasma (heparin).

  • The concentrations of CXCL1 were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: rat lung supernatant 1% and rat spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 57,709.68 pg/mL in neat rat lung supernatant and 822.66 pg/mL in neat rat spleen supernatant. The rat lung supernatant was cultured for six days in RPMI base media with 10% Fetal Bovine Serum (FBS) plus 5 µg/mL of LPS. The rat spleen supernatant was cultured for three days in RPMI base media with 10% FBS.

  • The concentrations of CXCL1 were measured in duplicate and interpolated from the CXCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 532.46 pg/mL in C6 cell extract.

Protocols

References

ab219044 has not yet been referenced specifically in any publications.

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