Rat CXCL3 ELISA Kit (ab213927)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich
- Reacts with: Rat
Overview
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Product name
Rat CXCL3 ELISA Kit
See all GRO gamma kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 40pg/ml 2.84 = 7.1% 2 16 190pg/ml 14.82 = 7.8% 3 16 394pg/ml 19.3 = 4.9% Inter-assay Sample n Mean SD CV% 1 24 43pg/ml 3.09 = 7.2% 2 24 206pg/ml 19.98 = 9.7% 3 24 390pg/ml 20.28 = 5.2% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich -
Sensitivity
< 10 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
The Rat CXCL3 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213927) is designed for the quantitative measurement of Rat CXCL3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL3 has been precoated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for CXCL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat CXCL3 amount of sample captured in plate.
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Notes
Chemokine (C-X-C motif) ligand 3 (CXCL3) is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2 in rat. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-rat CXCL3 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- rat CXCL3 antibody 1 x 100µl Lyophilized recombinant rat CXCL3 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 3
- C-X-C motif chemokine ligand 3
- Chemokine (C X C motif) ligand 3
see all -
Database links
- Entrez Gene: 171551 Rat
- SwissProt: Q10746 Rat
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab213927 has not yet been referenced specifically in any publications.