Key features and details
- Sensitivity: 80 pg/ml
- Range: 68.59 pg/ml - 50000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Product nameRat CXCL7 ELISA Kit (PPBP)
See all CXCL7 kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 80 pg/ml
Range68.59 pg/ml - 50000 pg/ml
> 100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 115.5 104% - 124% Serum 119.2 109% - 129% Plasma 116.3 93% - 132%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Rat
Abcam’s CXCL7 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CXCL7 in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Rat CXCL7 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CXCL7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-Rat CXCL7 2 vials CXCL7 Microplate (12 x 8 wells) 1 unit Recombinant Rat CXCL7 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceCXCL7 is a platelet-derived growth factor that belongs to the CXC chemokine family. It is a potent chemoattractant and activator of neutrophils and has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
- B TG1
- Beta TG
- Beta thromboglobulin
ab100781 has not yet been referenced specifically in any publications.