Rat Cytokine Antibody Array A (10 Targets) - Quantitative (ab197481)

Overview

  • Product name

    Rat Cytokine Antibody Array A (10 Targets) - Quantitative
    See all Cytokine Antibody Array antibody arrays
  • Sample type

    Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
  • Assay type

    Quantitative
  • Assay time

    6h 00m
  • Species reactivity

    Reacts with: Rat
  • Product overview

    ab197481 can be used for quantitative measurement of 10 Rat cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
    Targets names: CINC-2, CINC-3, CNTF, Fractalkine, GM-CSF, IL-1α, IL-4, LIX, β-NGF, VEGF.
    Learn more about quantitative antibody arrays

  • Notes

    Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins.

    Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc.

    For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation.

    Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader.

    Learn more about quantitative antibody arrays

    Learn more about membrane antibody arrays

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 8 tests 22 tests 50 tests
    20X Wash Buffer I 2 x 30ml 3 x 30ml 6 x 30ml
    20X Wash Buffer II 1 x 30ml 1 x 30ml 2 x 30ml
    Cy3 equivalent dye-conjugated Streptavidin 1 x 5µl 2 x 5µl 4 x 5µl
    Rat Cytokine Array A (10 T) Biotinylated Antibody Cocktail 1 unit 2 units 4 units
    Rat Cytokine Array A (10 T) Glass Slide 1 slide 2 slides 4 slides
    Rat Cytokine Array A (10 T) Lyophilized Cytokine Standard Mix 1 vial 2 vials 4 vials
    Sample Diluent 1 x 15ml 1 x 15ml 2 x 15ml
    Slide Washer/Dryer (30 mL Centrifuge Tube) 1 unit 1 unit 2 units
  • Research areas

Images

  • Rat Cytokine Antibody Array A (10 Targets) - Quantitative (ab197481) Assay Summary

  • Array Map for Rat Cytokine Antibody Array A (10 Targets) - Quantitative (ab197481).

  • Standard Curve obtained with Rat Cytokine Antibody Array A (10 Targets) - Quantitative (ab197481).

Protocols

References

ab197481 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

I can confirm that these arrays are provided on slides. So they will require a microarray scanner.
I can provide a list of suggested scanners:

Recommended Scanners
- InnoScan 710 Microarray Scanner (Innopsys)*
- InnoScan 900 Microarray Scanner (Innopsys)*
- GenePix® 4000A (Molecular Devices)
- GenePix® 4000B (Molecular Devices)
- GenePix® 4100A (Molecular Devices)
- GenePix® Professional 4200A (Molecular Devices)
- ScanArray® Lite (PerkinElmer, Inc.)
- ScanArray® Express (PerkinElmer
- ScanArray® Express HT (PerkinElmer)
- ScanArray® 4000 (PerkinElmer)
- ScanArray® 4000XL (PerkinElmer)
- ScanArray® 5000 (PerkinElmer)
- ScanArray® 5000XL (PerkinElmer)
- LS Series Laser Scanner (Tecan Group AG)
- AlphaScan Microarray Scanner (Alpha Innotech)
- The DNAscope LM (Biomedical Photometrics)
- The DNAscope IV & V (Biomedical Photometrics)
- Open Frame DNAscope (Biomedical Photometrics)
- Revolution 4200 Microarray Scanner (VIDAR)
- aQuire 110V (Genetix)
- aQuire 240V (Genetix)
- VersArray ChipReader 5um System (Bio-Rad)
- VersArray ChipReader 3um System (Bio-Rad)


Compatible Scanners
- NovaRay Detection Platform (Alpha Innotech)
- arrayWoRx (Applied Precision)
- GSD-501 System Calibration Kit (Invitrogen)
- DNA Microarray Scanner (Agilent Technologies**)
- Odyssey Imaging System (LiCor***)

Please note that this is not an exhaustive list. In general, most gene microarray scanners will be compatible as long as they have a Cy3 (green) channel, pixel resolution of 20um and able to scan a standard histology slide.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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