Overview

  • Product name

    Rat Dkk-1 ELISA Kit
    See all DKK1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Buffer 20 488pg/ml 7.9%
    Buffer 20 286pg/ml 5%
    Buffer 20 163pg/ml 11.2%
    Inter-assay
    Sample n Mean SD CV%
    Buffer 485pg/ml 5.1%
    Buffer 263pg/ml 10.6%
    Buffer 131pg/ml 14%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    26.1 pg/ml
  • Range

    39.1 pg/ml - 1250 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 98 100pg/ml - 800pg/ml
    EDTA Plasma 96 100pg/ml - 800pg/ml

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s Dkk-1 in vitro Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Dkk-1 in plasma, serum and culture fluids.

    A monoclonal antibody specific for Dkk-1 has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. A Dkk-1 polyclonal antibody is then added, incubated and washed. An HRP conjugated antibody is then added incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.

  • Notes

           Compound                    Cross reactivity                  
    rat Dkk-1             100%

    mouse Dkk-2

                 ≤0.03%

    mouse Dkk-4

                 ≤0.03%

    mouse Sgy-1

                 ≤0.03%

     

     

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab136938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab136938.

  • A parallelism experiment was carried out to determine if the recombinant Dkk-1 standard accurately measures Dkk-1 concentrations in biological matrices. To assess parallelism, values for serum were obtained from a standard curve using four parameter logistic curve fitting. The observed concentration was plotted against the dilution factor. Parallelism indicates antibody-binding characteristics of the native and standard proteins are similar, allowing accurate determination of analyte.

Protocols

References

ab136938 has not yet been referenced specifically in any publications.

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