RecombinantRabMAb

Rat Fas Antibody Pair - BSA and Azide free (ab256647)

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Sandwich ELISA - Rat Fas Antibody Pair - BSA and Azide free (ab256647)

    Key features and details

    • Unconjugated capture and detector antibodies
    • Adaptable to any antibody pair-based assay format
    • Antibody concentration ~ 1 mg/ml
    • BSA and azide free buffer - ready for conjugation
    • Reacts with: Rat

    Overview

    • Product name

      Rat Fas Antibody Pair - BSA and Azide free
      See all Fas kits

    • Assay type

      ELISA set

    • Range

      156.3 pg/ml - 10000 pg/ml

    • Species reactivity

      Reacts with: Rat

    • Product overview

      The Antibody Pair can be used to quantify Rat Fas. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


      For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab245706), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


      Download SDS here.

    • Tested applications

      Suitable for: Sandwich ELISAmore details

    • Platform

      Reagents

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.

    • Carrier free

      Yes
    • Components 10 x 96 tests
      Rat Fas Capture Antibody (unconjugated) 1 x 100µg
      Rat Fas Detector Antibody (unconjugated) 1 x 100µg

    • Research areas

    • Function

      Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).

    • Tissue specificity

      Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.

    • Involvement in disease

      Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.

    • Sequence similarities

      Contains 1 death domain.
      Contains 3 TNFR-Cys repeats.

    • Domain

      Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.

    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt

    • Alternative names

      • ALPS 1A
      • ALPS1A
      • APO 1
      • Apo 1 antigen
      • APO 1 cell surface antigen
      • Apo-1 antigen
      • APO1
      • Apo1 antigen
      • APO1 cell surface antigen
      • Apoptosis antigen 1
      • Apoptosis mediating surface antigen FAS
      • Apoptosis-mediating surface antigen FAS
      • APT 1
      • APT1
      • CD 95
      • CD 95 antigen
      • CD95
      • CD95 antigen
      • Delta Fas
      • Delta Fas/APO 1/CD95
      • Delta Fas/APO1/CD95
      • Fas
      • Fas (TNF receptor superfamily, member 6)
      • FAS 1
      • FAS 827dupA
      • Fas AMA
      • FAS Antigen
      • Fas cell surface death receptor
      • FAS1
      • FASLG receptor
      • FASTM
      • sFAS
      • Surface antigen APO1
      • TNF receptor superfamily, member 6
      • TNFRSF 6
      • TNFRSF6
      • TNR6_HUMAN
      • Tumor necrosis factor receptor superfamily member 6
      see all

    • Database links

      Associated products

      Applications

      The Abpromise guarantee

      Our Abpromise guarantee covers the use of ab256647 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      Sandwich ELISA
      Use at an assay dependent concentration.
      Notes
      Sandwich ELISA
      Use at an assay dependent concentration.

      Images

      • Sandwich ELISA - Rat Fas Antibody Pair - BSA and Azide free (ab256647)
        Sandwich ELISA - Rat Fas Antibody Pair - BSA and Azide free (ab256647)
        To learn more about the advantages of recombinant antibodies see here.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab256647? Please let us know so that we can cite the reference in this datasheet.

      ab256647 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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