Key features and details
- Sensitivity: 90 pg/ml
- Range: 82.3 pg/ml - 60000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Product nameRat Fas Ligand ELISA Kit (CD95L)
See all Fas Ligand kits
Sample typeCell culture supernatant, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 90 pg/ml
Range82.3 pg/ml - 60000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 92.64 81% - 98% Plasma 118.9 109% - 125%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Rat
Abcam’s Fas Ligand (CD95L) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Fas Ligand (CD95L) in plasma and cell culture supernatants. (Rat Fas Ligand concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Rat Fas Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Fas Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Rat Fas Ligand in normal serum with ab100759, only in serum spiked with Rat Fas Ligand.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 160X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-rat Fas Ligand 2 vials Fas Ligand Microplate (12 x 8 wells) 1 unit Recombinant Rat Fas Ligand Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceFas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system.
Cellular localizationCell Membrane (single-pass type II membrane protein) and Secreted
- Apoptosis (APO 1) antigen ligand 1
- Apoptosis antigen ligand
ab100759 has not yet been referenced specifically in any publications.