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|Sample type||Average %||Range|
|Cell culture supernatant||92.64||81% - 98%|
|Plasma||118.9||109% - 125%|
Abcam’s Fas Ligand (CD95L) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Fas Ligand (CD95L) in plasma and cell culture supernatants. (Rat Fas Ligand concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Rat Fas Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Fas Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Rat Fas Ligand in normal serum with ab100759, only in serum spiked with Rat Fas Ligand.
|Components||1 x 96 tests|
|160X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent C||1 x 30ml|
|Biotinylated anti-rat Fas Ligand||2 vials|
|Fas Ligand Microplate (12 x 8 wells)||1 unit|
|Recombinant Rat Fas Ligand Standard (lyophilized)||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100759 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"