Overview

  • Product name

    Rat Ferritin ELISA Kit
    See all Ferritin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type

    Serum, Plasma, Other biological fluids
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2.769 ng/ml
  • Range

    12.5 ng/ml - 400 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam's Ferritin Rat ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of ferritin in rat biological samples.


     


    In this assay the ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-conjugated anti-rat Ferritin antibody 1 x 150µl
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    Chromogen Substrate Solution 1 x 12ml
    Rat Ferritin Calibrator (Lyophilized) 1 vial
    Rat Ferritin ELISA Microplate 1 unit
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • Involvement in disease

    Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
  • Sequence similarities

    Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt
  • Alternative names

    • ferritin L chain
    • Ferritin L subunit
    • Ferritin light chain
    • ferritin light polypeptide
    • ferritin light polypeptide like 3
    • FRIL_HUMAN
    • FTH1
    • FTL
    • FTL1
    • NBIA3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab157732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab157732 Ferritin Rat ELISA Kit.

Protocols

References

This product has been referenced in:

  • Woodman AG  et al. Modest and Severe Maternal Iron Deficiency in Pregnancy are Associated with Fetal Anaemia and Organ-Specific Hypoxia in Rats. Sci Rep 7:46573 (2017). Read more (PubMed: 28440316) »
See 1 Publication for this product

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