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    rat-fibrinogen-elisa-kit-ab108845.pdf

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Cardiovascular Blood Fibrinolysis / Thrombolysis
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Rat Fibrinogen ELISA Kit (ab108845)

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  • SDS
  • Protocol Booklet
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Typical Standard Curve

    Key features and details

    • Sensitivity: 3 ng/ml
    • Range: 3.125 ng/ml - 200 ng/ml
    • Sample type: Cell culture supernatant, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Rat

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    Overview

    • Product name

      Rat Fibrinogen ELISA Kit
      See all Fibrinogen kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 4.9%
      Inter-assay
      Sample n Mean SD CV%
      Overall 7.2%
    • Sample type

      Cell culture supernatant, Urine
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      = 3 ng/ml
    • Range

      3.125 ng/ml - 200 ng/ml
    • Recovery

      96 %

    • Assay time

      3h 30m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Rat
    • Product overview

      Abcam’s Fibrinogen Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibrinogen in cell culture supernatant, and urine samples.


      A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibrinogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibrinogen captured in plate.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X Streptavidin-Peroxidase Conjugate 1 x 80µl
      10X Diluent N Concentrate 1 x 30ml
      20X Wash Buffer Concentrate 2 x 30ml
      50X Biotinylated Rat Fibrinogen Antibody 1 x 120µl
      Chromogen Substrate 1 x 7ml
      Fibrinogen Microplate (12 x 8 well strips) 1 unit
      Fibrinogen Standard 1 vial
      Sealing Tapes 3 units
      Stop Solution 1 x 11ml
    • Research areas

      • Cardiovascular
      • Blood
      • Fibrinolysis / Thrombolysis
      • Cardiovascular
      • Blood
      • Platelets
      • Cardiovascular
      • Blood
      • Coagulation
      • Common
      • Cardiovascular
      • Atherosclerosis
      • Thrombosis
      • Other
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      • Kits
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      • Metabolism
      • Types of disease
      • Metabolic disorders
    • Function

      Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
    • Tissue specificity

      Plasma.
    • Involvement in disease

      Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
      Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
    • Sequence similarities

      Contains 1 fibrinogen C-terminal domain.
    • Domain

      A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
    • Post-translational
      modifications

      The alpha chain is not glycosylated.
      Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
      About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
      Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
      Phosphorylation sites are present in the extracellular medium.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02671 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • FGA
      • FGB
      • FGG
      • Fib2
      • FIBA_HUMAN
      • Fibrinogen A alpha polypeptide
      • Fibrinogen alpha chain
      • Fibrinogen B alpha polypeptide
      • Fibrinogen beta chain
      • Fibrinogen G alpha polypeptide
      • Fibrinogen gamma chain
      • fibrinogen, B beta polypeptide
      • fibrinogen, G gamma polypeptide
      • fibrinogen, gamma polypeptide
      • Fibrinogen--alpha -polypeptide chain
      • Fibrinogen--beta -polypeptide chain
      • Fibrinogen--gamma-polypeptide chain
      see all
    • Database links

      • Entrez Gene: 361969 Rat
      • Entrez Gene: 24366 Rat
      • Entrez Gene: 24367 Rat
      • SwissProt: P14480 Rat
      • SwissProt: P02680 Rat
      • SwissProt: P06399 Rat
      • Unigene: 98846 Rat

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab108845

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab108845? Please let us know so that we can cite the reference in this datasheet.

      ab108845 has not yet been referenced specifically in any publications.

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