Rat Fibrinogen ELISA Kit (ab108845)
- Datasheet
- References
- Protocols
Overview
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Product name
Rat Fibrinogen ELISA Kit
See all Fibrinogen kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.9% Inter-assay Sample n Mean SD CV% Overall 7.2% -
Sample type
Cell culture supernatant, Urine -
Assay type
Sandwich (quantitative) -
Sensitivity
= 3 ng/ml -
Range
3.125 ng/ml - 200 ng/ml -
Recovery
96 %
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Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s Fibrinogen Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibrinogen in cell culture supernatant, and urine samples.
A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibrinogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibrinogen captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Rat Fibrinogen Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Fibrinogen Microplate (12 x 8 well strips) 1 unit Fibrinogen Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
Post-translational
modificationsThe alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracellular medium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- FGA
- FGB
- FGG
see all -
Database links
- Entrez Gene: 361969 Rat
- Entrez Gene: 24366 Rat
- Entrez Gene: 24367 Rat
- SwissProt: P14480 Rat
- SwissProt: P02680 Rat
- SwissProt: P06399 Rat
- Unigene: 98846 Rat
- Unigene: 11416 Rat
see all
Applications
Our Abpromise guarantee covers the use of ab108845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent dilution. |
Images
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Typical Standard CurveRepresentative Standard Curve using ab108845
Protocols
References
ab108845 has not yet been referenced specifically in any publications.
