Product nameRat GAP43 peptide
See all GAP43 proteins and peptides
Our Abpromise guarantee covers the use of ab16379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-GAP43 antibody - Neuronal Marker (ab16053)
ab16379 is the immunising peptide for Rabbit polyclonal to GAP-43 (ab16053) and can be used for blocking antibody binding in WB and IHC applications.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
- Axonal membrane protein GAP 43
- Axonal membrane protein GAP-43
- B 50
FunctionThis protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
Sequence similaritiesBelongs to the neuromodulin family.
Contains 1 IQ domain.
modificationsPhosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
Cellular localizationCell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab16379 has been referenced in 1 publication.
- Drobysheva A et al. Cardiac sympathetic innervation and PGP9.5 expression by cardiomyocytes after myocardial infarction: effects of central MR blockade. Am J Physiol Heart Circ Physiol 305:H1817-29 (2013). PubMed: 24124186