Key features and details
- Sensitivity: 78.1 pg/ml
- Range: 78.1 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Tissue Culture Media
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Rat
Product nameRat Gastrin 1 ELISA Kit
See all Gastrin I kits
Intra-assay Sample n Mean SD CV% Low 24 79.9pg/ml 8.3% Medium 24 260pg/ml 8.8% High 24 937pg/ml 8.5% Inter-assay Sample n Mean SD CV% Low 12 86.5pg/ml 14.7% Medium 12 233pg/ml 12.6% High 12 904pg/ml 7%
Sample typeCell culture supernatant, Serum, Plasma, Tissue Culture Media
Range78.1 pg/ml - 5000 pg/ml
Sample specific recovery Sample type Average % Range Serum 93 % - % Tissue Culture Media 92 % - % EDTA Plasma 95 % - %
Assay time3h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Rat
Abcam’s Gastrin 1 Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Gastrin 1 in tissue.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Gastrin I antigen and a polyclonal rabbit antibody specific to Gastrin I. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the coloration is inversely proportional to the amount of Gastrin I captured in the plate.
Gastrins are a family of sequence-related carboxyamidated peptides produced by endocrine G Cells of the antrum mucosa in response to a number of stimuli associated with digestion. Antral distension, partially digested proteins, amino acids, and vagal stimulation resulting from smelling, tasting, chewing or swallowing food all contribute to gastrin release from G Cell storage. In addition, caffeine, alcohol, hypoglycemia, antacids and elevated calcium levels will also stimulate gastrin release. Increased serum gastrin levels are associated with duodenal ulcers, Helicobacter pylori infections, colorectal carcinomas, and other tumors and cancerous lesions. Gastrin is the most potent stimulator of gastric acid secretion. Rat Gastrin I (G17) Pyr-Arg-Pro-Pro-Met-Glu-Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH2. Gastrin is synthesized as a 104 residue pre-pro-peptide on the rough endoplasmic reticulum, then post-translationally modified by cleavage and alpha-amidation to result in the active forms G34 and G17. Other forms also exist, but are not considered biologically significant. There are two types of G17 and G34, type II is sulfated at the tyrosine residue, while type I is not. Both G34 and G17 circulate and contribute to the stimulation of gastric acid secretion, but have different clearance rates. G34 is the major circulating Gastrin in fasting serum, but with G17, increases two to threefold after feeding until both are present in approximately equal amounts. Normal serum levels in rats have been reported over a range of 40-169 pg/mL with possible variations between animal sources.
Compound Cross Reactivity (%) Rat Gastrin 100 Cholecystokinin 26-33 (CCK-8) 100 Human Gastrin I 30 Mouse Gastrin I 20.5 Minigastrin (G13-I) 11 Gastrin Tetrapeptide (CCK-4) 0.75 Glucagon 0.5
Gastrin Inhibitory Polypeptide (GIP)
<0.2 Bombesin <0.2 Somatostatin-14 <0.2
Gastrin Releasing Peptide (GRP)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 30ml Assay Buffer 1 x 30ml Gastrin I Antibody 1 x 6ml Gastrin I Alkaline Phosphatase Conjugate 1 x 6ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 23ml Rat Gastrin I Standard 1 x 0.25ml Stop Solution 1 x 6ml
RelevanceGastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
- Big gastrin
ab133049 has been referenced in 1 publication.
- Na K & Kim HS Adrenalectomy abolishes hypergravity-induced gastric acid hyposecretion. Oncotarget 8:30700-30705 (2017). Functional Studies ; Rat . PubMed: 28430608